| 模拟微重力诱导PC12 细胞衰老的初步探讨 |
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| 引用本文： | 汪丹丹李呼伦王菁华刘怡希王广友孙博刘希君穆莉莉.模拟微重力诱导PC12 细胞衰老的初步探讨[J].现代生物医学进展,2011,11(1):52-55. |
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| 作者姓名： | 汪丹丹李呼伦王菁华刘怡希王广友孙博刘希君穆莉莉 |
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| 作者单位： | 哈尔滨医科大学神经生物学教研室,黑龙江省高校神经生物学重点实验室,黑龙江,哈尔滨,150081 |
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| 基金项目： | 黑龙江医药卫生科技计划项目(2009-206); 哈尔滨医科大学研究生创新基金(HCXB2008004) |
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| 摘 要： | 目的:构建模拟微重力条件下PC12细胞的培养体系,探讨模拟微重力对PC12细胞衰老的影响。方法:用Cytodex-3型微载体作为PC12细胞的贴附载体,旋转细胞培养系统所提供10-2g的微重力环境进行模拟微重力条件下的细胞培养。在倒置显微镜下观察PC12细胞的生长情况;用扫描电镜观察PC12细胞超微结构的变化;衰老相关β半乳糖苷酶(SA-β-gal)特异性染色对衰老的PC12细胞进行评估。结果:光镜下模拟微重力培养的PC12细胞表现出类衰老细胞的形态,扫描电子显微镜下观察发现其微绒毛增多。SA-β-gal染色的结果显示在模拟微重力的作用下,PC12细胞SA-β-gal的活性升高。结论:模拟微重力可以引起PC12细胞衰老样的形态变化,以及SA-β-gal的活性升高。
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| 关 键 词： | 细胞衰老 衰老相关β半乳糖苷酶 模拟微重力 PC12细胞 |
Influence of Simulated Microgravity on Cellular Senescence
of PC12 Cells |
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| WANG Dan-dan,LI Hu-lun,WANG Jing-hu,LIU Yi-xi,WANG Guang-you,SUN Bo,LIU Xi-jun,MU Li-li.Influence of Simulated Microgravity on Cellular Senescence
of PC12 Cells[J].Progress in Modern Biomedicine,2011,11(1):52-55. |
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| Authors: | WANG Dan-dan LI Hu-lun WANG Jing-hu LIU Yi-xi WANG Guang-you SUN Bo LIU Xi-jun MU Li-li |
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| Institution: | △(Department of Neurobiology,Harbin Medical University,Heilongjiang Provincial Key Laboratory of Neurobiology,150081,Harbin,China) |
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| Abstract: | Objective: To develop PC12 cell culture system under the condition of simulated microgravity and to investigate the influence of simulated microgravity on cellular senescence of PC12 cells.Methods: Cytodex-3 micro-carriers were used as the adherent carrier of PC12 cells.The microgravity environment was simulated by a rotary cell culture system.The growth of PC12 cells cultured on micro-carriers was detected by inverted microscope.The ultrastructure was detected by the scanning electron microscope.Senescence... |
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