| microRNA-194模拟物对成骨肉瘤细胞系SOSP_9607生物学
特性的影响 |
| |
| 引用本文： | 靳雷高杰刘云燕马琼杨彤涛裘秀春范清宇马保安.microRNA-194模拟物对成骨肉瘤细胞系SOSP_9607生物学
特性的影响[J].现代生物医学进展,2011,11(4):615-617. |
| |
| 作者姓名： | 靳雷高杰刘云燕马琼杨彤涛裘秀春范清宇马保安 |
| |
| 作者单位： | 第四军医大学唐都医院全军骨科中心暨全军骨肿瘤研究所,陕西,西安,710038 |
| |
| 基金项目： | 国家自然科学基金项目(No.30672143) |
| |
| 摘 要： | 目的:观察miR-194模拟物对于成骨肉瘤细胞系SOSP9607细胞增殖、周期和凋亡的影响。方法:四甲基偶氮唑蓝（MTT）法绘制细胞生长曲线,流式细胞仪测定细胞凋亡和周期。将SOSP9607细胞分为对照组和实验组,对照组分为阴性对照和正常细胞对照组。实验组采用miR-194模拟物（hsa-miR-194mimics）转染成骨肉瘤细胞系SOSP9607,增强SOSP9607细胞内miR-194的活性。结果:与对照组比较,实验组细胞的增殖能力明显下降。实验组凋亡率（10.1±0.22）%与阴性对照组凋亡率（3.3±0.19）%相比明显增高（P<0.01）。与对照组比较,实验组细胞周期G0/G1细胞比例显著增加,G2/M期细胞比例显著减少,S期细胞比例显著减少（P<0.01）。结论:通过转染miR-194模拟物增强SOSP9607细胞中miR-194的活性对SOSP9607细胞的增殖和凋亡造成显著影响。
|
| 关 键 词： | 骨肉瘤 成骨肉瘤细胞系SOSP9607 miR-194 细胞增殖 细胞凋亡 |
Effects of MicroRNA-194 mimics on Proliferation and Apoptosis
of Human Osteosarcoma Cell Line SOSP-9607 |
| |
| JIN Lei,GAO Jie,LIU Yun-yan,MA Qiong,YANG Tong-tao,QIU Xiu-chun,FAN Qing-yu,MA Bao-an.Effects of MicroRNA-194 mimics on Proliferation and Apoptosis
of Human Osteosarcoma Cell Line SOSP-9607[J].Progress in Modern Biomedicine,2011,11(4):615-617. |
| |
| Authors: | JIN Lei GAO Jie LIU Yun-yan MA Qiong YANG Tong-tao QIU Xiu-chun FAN Qing-yu MA Bao-an |
| |
| Institution: | JIN Lei,GAO Jie,LIU Yun-yan,MA Qiong,YANG Tong-tao,QIU Xiu-chun,FAN Qing-yu,MA Bao-an(Department of Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA,Tangdu Hospital,Fourth Military Medical University,Xi'an,Shanxi,710038,P.R.China) |
| |
| Abstract: | Objective:To investigate the effect of microRNA-194 mimics on proliferation,cell cycle and apoptosis of human os-teosarcoma cell line SOSP-9607.Methods:MTT assay was used to detect cell growth.FCM was employed to detect cell apoptosis rate and cell cycle analysis.Cultured SOSP-9607 cells were divided into three groups:negtive control group,blank group and experimental group.In experimental group,hsa-miR-194 mimics were used to increase the activity of miR-194.Results:Hsa-miR-194 mimics inhibit-ed SOSP-9607 ... |
| |
| Keywords: | Osteosarcoma SOSP-9607 cell line miR-194 Cell proliferation Cell apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《现代生物医学进展》浏览原始摘要信息 |
| 点击此处可从《现代生物医学进展》下载免费的PDF全文 |
|
