培养破骨细胞扫描电镜样本制备方法的探讨

目的:探讨体外培养的破骨细胞在自制牛股骨磨片和细胞爬片中扫描电镜制备方法。方法:实验分两组,一组采用新鲜牛股骨制备成5mm×5mm大小的薄片,作为共培养之需;另一组,采用盖玻片制成5mm×5mm的细胞爬片。分别以5×104种植于骨磨片和爬片,培养5天后进行扫描电镜的制备并观察。结果:破骨细胞在牛骨磨片表面生长良好,充分伸展,有细胞突起伸入到实验组材料深部,并形成骨陷窝;在爬片表面生长的破骨细胞,细胞生长良好,粘附性强,细胞之间相互连接较紧密,细胞表面突起明显。结论:牛股骨磨片与破骨细胞在体外相容良好,材料有利于破骨细胞的生长及细胞功能的表达,而破骨细胞爬片更适于细胞外形的观察。将两种方法结合既能反映破骨细胞的形态结构又能展示其破骨功能。

Discussion of growth of osteoclasts under scanning electron microscopy

Objective: o discuss scanning electron microscopy sample preparation of osteoclasts cultured in vitro in the self-made cell crawling and grinding beef femur. Methods: o discuss scanning electron microscopy sample preparation of osteoclasts cultured in vitro in the self-made cell crawling and grinding beef femur. was prepared with fresh bull stock size of 5 mm × 5 mm thin slices, as the need of co-culture. Another group, the use of 5mm × 5mm cover glass pieces made into cell growth, respectively, 5×104 cells bone grinding plant them. Cultured for 5 days for the preparation of scanning electron microscopy observed. Results: Osteoclasts are found in bone growth and a good grinding surface and fully extended, there are cell processes stretching deep into the study group materials and the formation of lacunae. Growth in the coverslip surface of osteoclasts, cells grew well and strong adhesion, closer connections between cells, cell surface protrusions evident. Conclusions: The grinding beef femur and osteoclasts have good compatibility in vitro. And the material is conducive to the growth of osteoclasts and the expression of cell function. The osteoclasts grown on coverslips are more suitable for observation of cell shape by scanning electron microscope. The combination of two methods not only reflects the morphology of osteoclasts but also demonstrates its Osteoclast function.