| 大鼠心肌梗死后差异蛋白质组学分析 |
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| 引用本文： | 张翠丽,许玲,王波,臧师竹,邹向阳,辛毅,姜涛.大鼠心肌梗死后差异蛋白质组学分析[J].现代生物医学进展,2011,11(2):230-232. |
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| 作者姓名： | 张翠丽 许玲 王波 臧师竹 邹向阳 辛毅 姜涛 |
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| 作者单位： | 1. 大连医科大学生物技术系,辽宁,大连,116044
2. 大连医科大学病理学与法医学教研室,辽宁,大连,116044 |
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| 摘 要： | 目的:建立大鼠心肌梗死后蛋白表达变化谱,以进一步了解心肌梗死后心肌细胞重塑产生机制。方法:通过结扎大鼠冠状动脉左前降支建立急性心肌梗死模型,利用蛋白质双向凝胶电泳技术(two-dimensionalgelelectrophoresis,2-DE)分离心肌总蛋白,采用PDQuest7.3.1软件比较分析,获得差异表达蛋白总体变化趋势。进一步通过蛋白免疫印记技术(Western-blotting)检测碱性成纤维生长因子(Basefibroblastgrowthfactor,bFGF)在心肌梗死后的表达变化。结果:成功建立了大鼠急性心肌梗死模型;2-DE结果表明:以假结扎组为对照,梗死3天组有27个蛋白显著上调,18个蛋白显著下调,7个蛋白表达明显差异(ratio>5)。进一步研究发现梗死区心肌组织bFGF表达明显升高。结论:心肌梗死后蛋白表达变化趋势的探讨为心室重塑机制研究提供线索。
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| 关 键 词： | 心肌梗死 双向电泳 蛋白印记 碱性成纤维生长因子 |
Differential Proteomic Analysis after Myocardial Infarction in Rats |
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| ZHANG Cui-li,XU Ling,WANG Bo,ZANG Shi-zu,ZOU Xiang-yang,XIN Yi,JIANG Tao.Differential Proteomic Analysis after Myocardial Infarction in Rats[J].Progress in Modern Biomedicine,2011,11(2):230-232. |
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| Authors: | ZHANG Cui-li XU Ling WANG Bo ZANG Shi-zu ZOU Xiang-yang XIN Yi JIANG Tao |
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| Institution: | 1△(1 Biotechnology Department of Dalian Medical University,Dalian 116044,China;2 Pathology Forensic Medicine Department of Dalian Medical University,Dalian 116044,China;) |
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| Abstract: | Objective:To investigate the mechanism of ventricle remodeling after myocardial infarction by analyzing the proteins detected by ampholyte pH gradients based two-dimensional gel electrophoresis(2-DE).Methods:The animal model of acute myocardial infarction was established by surgical legation of the left coronary artery in rat.The total proteins of normal and infracted cardiac muscle tissues were extracted.The differentially expression proteins were selected by using image analysis software.The expression of... |
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| Keywords: | Myocardial infarction Two-dimensional gel electrophoresis(2-DE) Western-blotting Base fibroblast growth factor |
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