| 电磁辐射对大鼠内皮祖细胞和肾脏组织学的影响 |
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| 引用本文： | 赵洪雯,张广斌,王源,杨学森,余争平.电磁辐射对大鼠内皮祖细胞和肾脏组织学的影响[J].现代生物医学进展,2011,11(2):262-266. |
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| 作者姓名： | 赵洪雯 张广斌 王源 杨学森 余争平 |
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| 作者单位： | 1. 第三军医大学西南医院肾科,重庆,400038
2. 第三军医大学预防医学院劳动卫生学教研室,重庆,400038 |
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| 基金项目： | 第三军医大学电磁辐射医学防护教育部重点实验室开放基金资助 |
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| 摘 要： | 目的:研究电磁辐射对体外培养骨髓来源的内皮祖细胞(EPCs)增殖、迁移、黏附能力的影响,并探讨其与肾脏疾病的可能关系。方法:密度梯度离心法获取大鼠骨髓单个核细胞(MNCs),接种至纤维连接素包被的培养板上,培养6d后进行免疫细胞化学和免疫荧光鉴定EPCs。采用MTT比色法、Transwell小室和黏附能力测定实验,观察平均功率密度为65mW/cm2,时间20min的电磁辐射对EPCs的增殖、迁移、黏附能力的影响;同等剂量全身照射大鼠,光镜和透射电镜观察大鼠肾脏组织学和超微结构的变化。结果:从大鼠骨髓能成功分离培养获得EPCs。EPCs的增殖、迁移、黏附能力较对照组显著下降;大鼠接受全身照射后各时相点无明显组织学改变,但超微结构显示在照射后3h后开始出现肾小球毛细血管袢足突肿胀,12h后出现部分足突融合。结论:电磁辐射导致EPCs生物功能显著减弱,肾小球超微结构改变,电磁辐射可能与起肾脏疾病的发生有关。
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| 关 键 词： | 内皮祖细胞 电磁辐射 肾脏病 |
The Effects of Electromagnetic Radiation on Endothelial Progenitor
Cells and Renal Histology |
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| ZHAO Hong-wen,ZHANG Guang-bin,WANG Yuan,YANG Xue-sen,YU Zheng-ping.The Effects of Electromagnetic Radiation on Endothelial Progenitor
Cells and Renal Histology[J].Progress in Modern Biomedicine,2011,11(2):262-266. |
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| Authors: | ZHAO Hong-wen ZHANG Guang-bin WANG Yuan YANG Xue-sen YU Zheng-ping |
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| Institution: | 1 Department of Kidney,Southwest Hospital Chongqing,400038 China;2 Department of Occupational Hygiene,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038,China) |
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| Abstract: | Objective:To investigate the effects of electromagnetic radiation on proliferation,migration,adhesion of endothelial progenitor cells(EPCs) cultured in vitro derived from rats bow marrow,and to investigate the relationship between electromagnetic radia-tion and kidney disease.Methods:Mononuclear cells(MNCs) were obtained from rats' bone marrow by density gradient centrifugation,cultured with EGM-2 complete medium on plate coated by fibronectin.After 6 days,the cells were identified by immunocytochemistry an... |
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| Keywords: | Endothelial Progenitor Cells(EPCs) Electromagnetic Radiation Kidney Disease |
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