双氢青蒿素调控巨噬细胞增殖和迁移的作用研究

目的:探讨双氢青蒿素在体外对小鼠单核巨噬细胞RAW264.7的增殖、克隆形成、周期、凋亡和迁移的影响。方法:采用梯度浓度(2.5μg/m L, 5μg/m L, 10μg/m L, 20μg/m L)的双氢青蒿素处理RAW264.7细胞,利用CCK8实验检测双氢青蒿素对巨噬细胞增殖能力的影响,利用克隆形成实验检测双氢青蒿素对RAW264.7细胞克隆形成能力的影响,利用流式细胞术检测双氢青蒿素对RAW264.7细胞周期和凋亡的影响,利用划痕修复实验检测RAW264.7细胞迁移能力。结果:CCK8实验结果显示,双氢青蒿素可以显著抑制RAW264.7巨噬细胞的增殖能力,且抑制效果与双氢青蒿素的浓度呈正相关性。克隆形成实验结果显示,双氢青蒿素可以抑制细胞的克隆形成能力。双氢青蒿素处理使RAW264.7细胞G0/G1期比例显著升高,S期与G2/M期细胞比例显著降低。双氢青蒿素对巨噬细胞凋亡具有诱导作用,且凋亡诱导作用呈现浓度依赖的特性。划痕修复实验结果显示,双氢青蒿素可以显著抑制RAW264.7巨噬细胞的迁移能力。结论:双氢青蒿素可以导致巨噬细胞的细胞周期G0/G1阻滞,并且诱导细胞凋亡,对巨噬细胞增殖和迁移具有抑制作用。

The Effect of Dihydroartemisinin on Macrophage Cells Proliferation and Migration

ABSTRACT Objective: To investigate the effect of dihydroartemisinin (DHA) on biological behavior of mouse macrophage cell, including cells proliferation, clone formation, cell cycle, apoptosis and migration. Methods: RAW264.7 cells were treatment with gradient concentration of DHA. The effect of DHA on macrophage cells proliferation was detected by CCK8. Clone formation assay was used to evaluate the effect of DHA on macrophage cells cloning ability. Cell apoptosis and cell cycle were examined by flow cytometry. And scratch assay was used to detect the effect of DHA on macrophage cells migration. Results: DHA significantly inhibited the proliferation of RAW264.7 cells in a dose and time dependent manner. And moreover, DHA could also suppressed the clone formation ability of RAW264.7 cells. The flow cytometry analysis showed that DHA treatment lead to markedly elevate the proportion of cells in G0/G1 period. The flow cytometry analysis showed that DHA significantly induce RAW264.7 cell apoptosis in a dose dependent manner. Moreover, DHA treatment could decrease the migration capabilities of RAW264.7 cells. Conclusion: DHA treatment can suppress RAW264.7 cells proliferation and migration. And moreover, DHA induce cell apoptosis and cell cycle G0/G1 arrest.