线粒体DNA4977bp大片缺失突变与喉癌的相关性研究

目的:探讨线粒体DNA4977bp大片缺失突变与喉癌的相关性。方法:选择2016年1月～2017年6月我院收治的喉乳头状瘤、喉癌患者,分别纳入良性肿瘤组、恶性肿瘤组,每组各150例。取两组患者的病变组织标本,分离癌及癌旁组织,提取总DNA,采用PCR扩增测序技术检测两组标本中线粒体DNA4977bp大片缺失突变情况。结果:基因测序结果显示恶性肿瘤组患者的线粒体DNA4977bp缺失突变率为39.33%,高于良性肿瘤组患者的1.33%,差异具有统计学意义(P0.05)。不同肿瘤分期患者的线粒体DNA4977bp缺失突变率比较,差异具有统计学意义(P0.05),且III期患者的突变率II期 I期 IV期;淋巴结转移患者的线粒体DNA4977bp缺失突变率高于淋巴结未转移患者差异具有统计学意义(P0.05)。结论:线粒体DNA4977bp大片缺失突变与喉癌的发生有关,可能促进的发生和进展。

A Study on the Relationship between Mitochondrial DNA 4977bp Deletion Mutation and Laryngeal Cancer

ABSTRACT Objective: To investigate the relationship between mitochondrial DNA 4977bp deletion mutation and laryngeal cancer. Methods: From January 2016 to June 2017, patients with laryngeal papilloma and laryngeal cancer were enrolled into benign tumor group and malignant tumor group, 150 cases in each group. The total DNA was extracted from the diseased tissues of the two groups. The deletion mutation of mitochondrial dna4977bp was detected by PCR amplification sequencing. Results: The results of gene sequencing showed that the mutation rate of mitochondrial dna4977bp deletion in patients with malignant tumors was 39.33%, higher than that in patients with benign tumors (1.33%), the difference was statistically significant (P<0.05). Compared with the patients with different tumor stages, the difference was statistically significant (P<0.05), and the mutation rate of stage III patients was more than stage II > stage I > stage IV; the difference between the patients with lymph node metastasis and the patients without lymph node metastasis was statistically significant (P<0.05). Conclusion: The deletion mutation of mitochondrial dna4977bp may be related to the occurrence and progression of laryngeal cancer, but the specific mechanism needs to be further explored.