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Id 基因在多种肺癌细胞中的表达及意义
引用本文:李晓莉??,赵艳滨??,孙立春??,李乐静.Id 基因在多种肺癌细胞中的表达及意义[J].现代生物医学进展,2006,6(2):36-38.
作者姓名:李晓莉??  赵艳滨??  孙立春??  李乐静
作者单位:哈尔滨医科大学附属肿瘤医院内科,黑龙江,哈尔滨,150040
摘    要:目的:研究Id基因在肺癌和永生化支气管上皮细胞中的表达,探讨其在肺癌细胞中表达的意义。方法:利用半定量RT—PCR和Western blot方法检测多种肺癌和永生化支气管上皮细胞中Id1—Id4 mRNA和Id1—Id4蛋白的表达。结果:A549、NCI—H460、NCI—H446、SK—MES—1、Anip973中Id1-Id3 mRNA均高表达,Id1相对表达较强;而AGZY和MP-184中未表达Id1-Id3 mRNA;腺癌细胞均表达了Id4 mRNA,而NCI-H446、SK—MES—1未表达Id4mRNA。A549,NCA—H460,NCA—H446,SK—MES—1,Anip973中Id1,Id2,Id3蛋白均高表达,A549,NCA—H446,Anip973中Id2的表达高于NCA—H460,SK—MES—1;A549,NCA—H460,Anip973有出的高表达,NCI—H446,SK—MES—1无Id4的表达,Id1-Id4在AGZY和MP-184中均耒表达。结论:4种Id基因均作为癌基因在肺癌的发生发展中发挥作用,Id1,Id2,Id3与肺癌细胞的恶性程度以及增殖和转移密切相关,Id4可做为肺腺癌的检测标志物。

关 键 词:肺癌  Id基因  癌基因
收稿时间:2006-02-18
修稿时间:2006-03-12

The expression and significance of Id in different lung cancer cells
LI Xiao- li,ZHAO Yan- bin,SUN Li- chun,LI Le - j ing.The expression and significance of Id in different lung cancer cells[J].Progress in Modern Biomedicine,2006,6(2):36-38.
Authors:LI Xiao- li  ZHAO Yan- bin  SUN Li- chun  LI Le - j ing
Abstract:Objective: To investigate the expression and significance of Id gene in lung cancer and immortalization bronchial epithelial cells.Methods: The expressions of Id1-Id4 mRNA and their proteins were examined by semiquantitative RT-PCR and Western blot assay.Results: Id1 to Id3 mRNA were high expressed in A549,NCI-H460,NCI-H446,SK-MES-1and Anip973 cells but not in AGZY and MP-184 cells.The expression of Id1 was much higher than others.Id4 mRNA was expressed in adenocarcinoma cells but not in NCI-H446 and SK-MES-1 cells.The proteins of Id1 to Id3 were high expressed in A549,NCI-H460,NCI-H446,SK-MES-1 and Anip973 cells.The expression of Id2 in A549,NCI-H446 and Anip973 was higher than that in NCI-H460 and SK-MES-1.Id4 was high expressed in A549,NCI-H460 and Anip973 cells but not in NCI-H446 and SK-MES-1 cells.There were no expressions from Id1 to Id4 in AGZY and MP-184 cells.Conclusion: The four kinds of Id gene took a part in the progression and metastasis of lung cancer as an oncogene.Id1,Id2 and Id3 were closely related to the malignant degree,proliferation and metastasis of lung cancer.Id4 may be a mark of lung adenocarcinoma.
Keywords:Lung caner  Id gene  Oncogene
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