| 耐三苯氧胺乳腺癌异种移植瘤动物模型建立与评价 |
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| 引用本文： | 谌海燕,侯丽,陈信义.耐三苯氧胺乳腺癌异种移植瘤动物模型建立与评价[J].现代生物医学进展,2011,11(5):841-843. |
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| 作者姓名： | 谌海燕 侯丽 陈信义 |
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| 作者单位： | 1. 北京中医药大学东直门医院,北京,100700
2. 北京市与教育部重点实验室(中医内科学),北京,100700 |
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| 基金项目： | 北京中医药大学自主创新课题 |
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| 摘 要： | 目的:建立耐三苯氧胺(TAM)人乳腺癌的裸鼠移植瘤模型,为研究和治疗乳腺癌对TAM耐药提供研究工具。方法:采用雌激素受体阳性,对TAM耐药的人乳腺癌细胞系LCC2,接种于BALB/c裸鼠皮下,观察肿瘤生长趋势,用免疫组化方法进行鉴定。结果:在接种细胞数大于5×106/只时,Matrigel能够显著促进移植瘤的生长。肿瘤组织病理学检测证实为浸润性导管癌,且Pgp和Her-2为阳性表达。结论:该方法建立的耐TAM人乳腺癌移植瘤模型,周期短,成瘤率高,保留了与细胞系相同的肿瘤生物学特征。
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| 关 键 词： | 乳腺癌 移植瘤 三苯氧胺 |
Establishment of xenograft model in nude mice of
tamoxifen -resistanthuman breast cancer |
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| CHEN Hai-yan,HOU Li,CHEN Xin-yi.Establishment of xenograft model in nude mice of
tamoxifen -resistanthuman breast cancer[J].Progress in Modern Biomedicine,2011,11(5):841-843. |
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| Authors: | CHEN Hai-yan HOU Li CHEN Xin-yi |
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| Institution: | CHEN Hai-yan1,HOU Li1,CHEN Xin-yi2(1Dongzhimeng Hospital,Beijing University of Chinese Medicine,Beijing 100700,China,2The Key Laboratory of Beijing (Traditional Chinses Physician),China) |
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| Abstract: | Objective:This study aims to establish the xenograft model of human breast cancer resistant to tamoxifen(TAM)so as to provide a research tool for the study and treatment of breast cancer resistant to TAM.Methods:This study employs the method of inoculating the human breast cancer cells LCC2 which is estrogen receptor positive and resistant to TAM into BALB/c nude mice.Then it observes the trends of tumor growth,and identifies by immunohistochemistry methods.Results:When the number of inoculation cells is mo... |
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| Keywords: | Breast Cancer Xenograft Tamoxifen |
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