| 抗SEA鸡卵黄抗体用于血吸虫循环抗原检测的初步研究 |
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| 作者单位： | 滨州医学院病原生物学教研室，南京医科大学卫生部抗体技术重点实验室，华中科技大学同济医学院人体寄生虫学教研室 |
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| 摘 要： | 目的:探讨抗可溶性虫卵抗原(SEA)鸡卵黄抗体(Immunoglobulin Y,IgY)用于血吸虫循环抗原检测的可行性。方法:以纯化的鸡抗SEA卵黄抗体为捕捉抗体,以酶标抗SEA单克隆抗体NP28-5B进行双抗体夹心酶联免疫吸附试验法(S-ELISA)检测急、慢性血吸虫病病人和健康人血清,并与常规检测抗体的酶联免疫吸附试验(SEA-ELISA)法做比较。结果:S-ELISA法测得SEA浓度(y)与A450值(x)呈明显的正相关(r=0.9481,y=459.22x-108.14)。S-ELISA法检测急性血吸虫病人循环抗原的阳性率为100%,慢性血吸虫病人循环抗原的阳性率为84.40%,健康人的特异性为96%。两种方法对血吸虫病的检出率差异无统计学意义(P>0.05)。结论:S-ELISA可用于血吸虫病的免疫诊断。
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| 关 键 词： | 血吸虫 免疫诊断 循环抗原 IgY |
Detection of Circulating Schistosome Antigen with Anti-Sea Chicken Immunoglobulin Y |
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| Authors: | WU Yu-long ZHANG Wei-ming LIU Wen-qi QIU Zhen-ning FENG Zhen-qing LI Yong-long GUAN Xiao-hong |
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| Institution: | WU Yu-long~1 ZHANG Wei-ming~2 LIU Wen-qi~3 QIU Zhen-ning~2 FENG Zhen-qing~2 LI Yong-long~3 GUAN Xiao-hong~2 |
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| Abstract: | Objective:To analyze the feasibility on detection of circulating schistosome antigen with anti-SEA chicken im- munoglobulin Y (IgY).Methods:Chicken anti-SEA IgY was used as the capture-antibody and an anti-SEA mouse mAb conjugated with HRP as the detection antibody,a sandwich ELISA technique was established to detect the circulating schistosome antigen in serum sam- ples of patients with acute,chronic schistosomiasis and healthy people.The results were compared with SEA-ELISA.Results:There was an obviously positive correlation between SEA concentration and A450 value.The regression equation was deduced (r=0.9481,y=459. 22x-108.14).The positive rates of S-ELISA for testing acute,chronic schistosomiasis patients were 100% and 84%,respectively.Its specificity for healthy people was 96%.No significant difference was observed between the two ELISA methods.Conclusion:S-ELISA could be used in immunodiagnosis of schistosomiasis. |
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| Keywords: | Schistosome Immunodiagnosis Circulatingantigen IgY |
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