固液态发酵条件对桑叶发酵提取物中降糖活性物质的影响

目的:探讨不同发酵条件对桑叶总黄酮、总多酚含量及体外降糖活性的影响。方法:本研究主要利用比色法考察固态、液态两种发酵条件对桑叶发酵物中总黄酮、总多酚含量的影响,同时建立α-葡萄糖苷酶和α-淀粉酶为体外降糖活性筛选模型,以期筛选最佳发酵条件。结果:液体发酵7 d桑叶70%乙醇提取物对α-葡萄糖苷酶抑制率最高(92.79%);未发酵水提取物对α-淀粉酶抑制率最高(63.68%),其次为液体发酵7 d桑叶70%乙醇提取物(54.81%)。且在生药浓度为10 mg·m L-1时,都高于阳性对照阿卡波糖。结论:利用冠突散囊菌发酵,可以改变桑叶提取物的体外降糖活性,为开发糖尿病药物提供可行思路。

Effect of Solid-state and Submerged Fermentation Conditions on Hypoglycemic Active Substances of Mulberry Leaf

Objective:To investigate the influence of different fermentation conditions on the total flavonoids content , total polyphenol content and vitro hypoglycemic activities from mulberry leaves.Methods:The influence of solid-state fermentation and submerged fermentation conditiongs was investigated for the total flavonoids content and tal polyphenol content of mulberry leaf extractions, and the vitro hypoglycemic active substance screening models of alpha-glucosidase and alpha-amylase were established so as to screen the best fermentation condition.Results:The 70% ethanol extraction by submerged fermented 7 d showed the highest alpha-glucosidase inhibitory activity which was 92.79%; and the water extraction of non-fermentation had higher alpha-amylase inhibitory activity which was 63.68%than the 70%ethanol extraction by submerged fermented 7 d which was 54.81%. At the same time, they were all higher than that of acarbose when the drug concentration was 10 mg·mL-1Conclusion:Fermentation by eurotimcristatum can change the vitro hypoglycemic activity of extract frommulberry leaves, which povides a feasible way for the development of drugs for diabetes.