Chitosan-DTPA-Gd舌间质磁共振淋巴造影显示兔颈淋巴的研究

目的：探讨磁共振间质淋巴造影剂Chitosan-DTPA-Gd经舌黏膜下注射后,显示颈淋巴的应用价值。方法：选取健康成年纯种新西兰大白兔12只,麻醉并仰卧位固定磁共振平扫,再于每只兔双侧舌缘中后交界处粘膜下各注射0.1 mL造影剂,注射部位按摩30秒,按摩后5、10、15、20、25、30、35、40 min行三维增强磁共振淋巴造影成像,测量增强前后不同时间颈部淋巴结的信号强度（SI）,计算相对应的信号强化率（E%）,采用SPSS软件进行数据的统计学分析。结果：Chitosan-DTPA-Gd于兔双侧舌缘粘膜下注射后很快吸收进入颈部淋巴引流区,淋巴结及淋巴管明显、均匀的强化,血管无显影。颈部淋巴结于注射后15 min信号强度达到峰值,并可保持一段时间,25 min后信号强化率开始明显降低,40分钟后淋巴系统显影与周围组织无差别。结论：间质磁共振淋巴造影剂Chitosan-DTPA-Gd用量小,强化效应明显,能够有效的显示颈部淋巴结形态及淋巴管走形。

Study on the Tongue Interstitial Magnetic Resonance with Chitosan-DTPA-Gd on Rabbit's Cervical Lymphography

Objective： To investigate the application of the magnetic resonance interstitial lymphoid contrast agent Chitosan-DTPA-Gd through the tongue submucosa injection for showing cervical lymph.Methods： 0.1 ml contrast agent was injected into the bilateral tongue margin submucosa of 12 healthy New Zealand rabbits,which were anesthesia and supine fixed and then the injection site was massaged for 30 seconds.MR images were obtained before injection and 5,10,15,20,25,30,35,40 minutes after injection by the 3D enhancement magnetic resonance lymphography.The signal intensities before and after enhancing of cervical lymph node were measured to calculate the enhancing rates（E %） by using SPSS11.5 software.Results： After injected into the rabbit＇s bilateral tongue margin submucosa,Chitosan-DTPA-Gd was absorbed quickly into the area of cervical lymphatics.From the magnetic resonance image,we can see lymph nodes and lymphatic vessels were strengthened significantly and evenly without blood vessels developing.The signal intensity of the cervical lymph nodes reached the peak within 15 min after injection,maintained a high level over a period of time,decreased after 25 min and would be undifferentiated with the surrounding tissues after 40 minutes.Conclusions： The interstitial magnetic resonance lymphoid contrast agent Chitosan-DTPA-Gd has significant strengthening effect with small dosage.It can effectively show the cervical lymph node morphology and lymphatic shape.