| pcDNA3.1-Canstatin-3Flag真核表达载体的构建及转染HepG2细胞中的表达 |
| |
| 引用本文： | 赵贵先,张志会,翟顺生,傅文达,张建华,王雪雯.pcDNA3.1-Canstatin-3Flag真核表达载体的构建及转染HepG2细胞中的表达[J].现代生物医学进展,2010,10(3). |
| |
| 作者姓名： | 赵贵先 张志会 翟顺生 傅文达 张建华 王雪雯 |
| |
| 作者单位： | 石河子大学医学院病理生理学教研室/新疆地方与民族高发病教育部重点实验室,新疆,石河子,832002 |
| |
| 基金项目： | 石河子大学“自然科学与技术创新”项目资助(ZRKX2008076) |
| |
| 摘 要： | 目的:构建pcDNA3.1-Canstatin-3Flag载体并稳定转染肝癌HepG2细胞,检测canstatin在mRNA水平的表达。方法:胎盘中提取总RNA,RT-PCR法获得canstatinDNA,克隆至pcDNA3.1(-)载体中,并测序,重组质粒pcDNA3.1-Canstatin-3Flag转染肝癌HepG2细胞,G418筛选出稳定转染细胞,RT-PCR检测canstatin mRNA表达。结果:1.成功构建出pcDNA3.1-Canstatin-3Flag重组质粒;2.获得稳定转染pcDNA3.1-Canstatin-3Flag的肝癌HepG2细胞;3.发现转染后的肝癌HepG2细胞canstatin在mRNA水平比未转染细胞有明显的增强。结论:获得了稳定转染pcDNA3.1-Canstatin-3Flag的肝癌HepG2细胞,为后期canstatin在肝癌中的研究提供了支持。
|
| 关 键 词： | canstatin 肝癌HepG2细胞 基因转染 RT-PCR |
pcDNA3.1-Canstatin-3Flag Eukaryotic Expression Vector:Its construction and expression in transfection of hepatocellular carcinoma HepG-2 cells |
| |
| ZHAO Gui-xian,ZHANG Zhi-hui,ZHAI Shun-sheng,FU Wen-da,ZHANG Jian-hua,WANG Xue-wen.pcDNA3.1-Canstatin-3Flag Eukaryotic Expression Vector:Its construction and expression in transfection of hepatocellular carcinoma HepG-2 cells[J].Progress in Modern Biomedicine,2010,10(3). |
| |
| Authors: | ZHAO Gui-xian ZHANG Zhi-hui ZHAI Shun-sheng FU Wen-da ZHANG Jian-hua WANG Xue-wen |
| |
| Institution: | ZHAO Gui-xian,ZHANG Zhi-hui,ZHAI Shun-sheng,FU Wen-da,ZHANG Jian-hua,WANG Xue-wen (Department of Pathology,Medical School of Shihezi University,Xinjiang Shihezi 832002) |
| |
| Abstract: | Objective:To construct pcDNA3.1-Canstatin-3Flag eukaryotic expression vector and stable transfection into hepatocellular carcinoma cell line HepG-2,and detect canstatin mRNA expression. Methods:The placenta extract total RNA, RT-PCR method to obtain canstatinDNA, cloned into pcDNA3.1 (-) vector and sequencing, recombinant plasmid pcDNA3.1-Canstatin-3Flag transfected hepatoma HepG2 cells, G418 selection a stable transfer transfected cells, RT-PCR detection of canstatin mRNA expression. Results:1. Successfull... |
| |
| Keywords: | Canstatin hepatocellular carcinoma cell line HepG-2 Gene transfection RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
