| 汉滩病毒感染血管内皮细胞后上调HLAI类分子的表达 |
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| 引用本文： | 于海涛,白雪帆,南雪平,张野,李彧,蒋伟,刘博,丁艳荣,姜泓,张颖,王平忠.汉滩病毒感染血管内皮细胞后上调HLAI类分子的表达[J].现代生物医学进展,2010,10(1). |
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| 作者姓名： | 于海涛 白雪帆 南雪平 张野 李彧 蒋伟 刘博 丁艳荣 姜泓 张颖 王平忠 |
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| 作者单位： | 第四军医大学唐都医院全军感染病中心,陕西,西安,710038 |
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| 基金项目： | 国家自然科学基金资助项目(30901346) |
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| 摘 要： | 目的:观察汉滩病毒感染血管内皮细胞(EVC304)前后,HLA-I类分子中HLA-A、HLA-B、HLA-Cw3、HLA-Cw5表达上的差异。方法:以汉滩病毒76-118株感染EVC304细胞作为实验组,以无任何刺激的EVC304细胞作为阴性对照组。6h后用RT-PCR的方法对HLA-A、HLA-B、HLA-Cw3、HLA-Cw5的mRNA进行反转录和基因片段的扩增,在mRNA水平上检测其表达差异。结果:在HTNV感染以后,EVC304细胞中HLA-A、HLA-B、HLA-Cw3、HLA-Cw5mRNA表达均呈升高现象,其中HLA-A、HLA-B和HLA-Cw5的mRNA表达与未感染细胞相比明显升高。结论:HTNV感染血管内皮细胞后,可以诱导HLA-A、HLA-B、HLA-Cw3、HLA-Cw5的表达升高,从而诱发机体特异性免疫应答,导致免疫损伤的发生。这或许是肾综合症出血热的发病机制之一。
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| 关 键 词： | 汉滩病毒 HLAI HLA-A HLA-B HLA-Cw3 HLA-Cw5 |
HLA I up-regulation in EVC304 cells infected by HTNV |
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| YU Hai-tao,BAI Xue-fan,NAN Xue-piag,ZHANG Ye,LI Yu,JIANG Wei,LIU Bo,DING Yan-rong,JIANG Hong,ZHANG Ying,WANG Ping-zhong.HLA I up-regulation in EVC304 cells infected by HTNV[J].Progress in Modern Biomedicine,2010,10(1). |
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| Authors: | YU Hai-tao BAI Xue-fan NAN Xue-piag ZHANG Ye LI Yu JIANG Wei LIU Bo DING Yan-rong JIANG Hong ZHANG Ying WANG Ping-zhong |
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| Institution: | YU Hai-tao,BAI Xue-fan,NAN Xue-ping,ZHANG Ye,LI Yu,JIANG Wei,LIU Bo,DING Yan-rong,JIANG Hong,ZHANG Ying,WANG Ping-zhong(Center of Infectious Diseases Tangdu Hospital,the Fourth Military Medical University,Xi\'an 710038,China) |
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| Abstract: | Objective:To investigate the expression of HLA I in EVC304 cells before and after Hantaan virus(HTNV) infection.Method:EVC304 cells infected by HTNV 76-118 were selected as the treated group,the cells without stimulation were selected as the negative control group.RT-PCR was used to detect the mRNA expression of HLA I.Result:After EVC304 cells were infected by HTNV,The expressions of HLA-A,HLA-B,HLA-Cw3,HLA-Cw5 were higher,and the expresssion of HLA-A,HLA-B and HLA-Cw5 increased distinctly.Conclusion:The ex... |
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| Keywords: | Hantaan virus(HTNV) HLA I HLA-A HLA-B HLA-Cw3 HLA-Cw5 |
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