1950 MHz射频电磁场对SRA01/04细胞周期和凋亡的影响

目的：研究显示射频电磁场与白内障的发生关系密切，为了评价晶状体上皮细胞在射频电磁场诱导的白内障发生中的作 用，本实验探讨了1950 MHz射频电磁场暴露对人眼晶状体上皮细胞株（SRA01/04）细胞周期与凋亡的影响。方法：将处于对数生 长期的SRA01/04 细胞暴露或假暴露于频率为1950 MHz，比吸收率（SAR）为2.79 W/kg 的射频电磁场中，每天暴露1 h，每周暴露 5 天，连续暴露4 周。暴露结束后立即收集细胞，显微镜下观察细胞形态变化，噻唑蓝（MTT）法检测细胞存活力，流式细胞仪 （FCM）检测细胞周期与凋亡。结果：与假辐照组相比，暴露组细胞形态未见明显变化；细胞存活力、细胞周期分布及细胞凋亡率亦 无显著改变（P>0.05）。结论：1950 MHz射频电磁场暴露4 周对SRA01/04 细胞的形态、活力、周期以及凋亡均无明显影响，提示在 本实验条件下1950 MHz 射频电磁场不会诱发白内障的发生。

Effects of 1950 MHz Radiofrequency Electromagnetic Fields on Cell Cycle and Apoptosis in SRA01/04 Cells

Objective:To investigate the effects of 1950 MHz radiofrequency electromagnetic fields on cell cycle and apoptosis in human lens epithelial (SRA01/04) cells and clarify the role 1950MHz radiofrequency electromagnetic fields plays in the cataractogenesis.Methods:SRA01/04 cells in exponential growth phase were exposed or sham-exposed to 1950 MHz radiofrequency electromagnetic fields at a specific absorption rate (SAR) of 2.79 W/kg for 1 hour one day, 5 days every week. After 4 weeks exposure, cells were collected instantly. The cell morphology was observed under invert microscope, and the cell viability was detected by MTT assay. Cell cycle and apoptosis of SRA01/04 cells were examined by flow cytometry.Results:Compared with sham-exposed cells, the shape of exposed cells showed no obvious change. Moreover, the cellular viability, cell cycle distribution and apoptosis between exposure group and sham group also showed no significant difference (P>0.05).Conclusions:The results suggested that 4-weeks 1950 MHz radiofrequency electromagnetic fields exposure could not affect cellular morphology, viability, cell cycle and apoptosis in SRA01/04 cells, these data suggested that 1950 MHz radiofrequency electromagnetic fields couldn''t induce cataract under this experimental condition.