蒙药乌力吉-18对大鼠下丘脑-垂体-卵巢轴相关激素及受体表达的影响

目的:探讨蒙药乌力吉-18对大鼠下丘脑-垂体-卵巢轴相关激素及受体的影响。方法:选取40只健康雌性未孕SD大鼠,随机分为空白组、对照组、乌力吉-18高、低2个剂量组,每组10只。空白组灌胃等体积蒸馏水,对照组灌胃逍遥丸,高、低剂量组分别灌胃2.0 g·kg^-1·d^-1、1.0 g·kg^-1·d^-1乌力吉-18,连续给药31学艺术d。采用酶联免疫吸附法测定血清促性腺激素释放激素(GnRH)、促卵泡生成素(FSH)、黄体生成素(LH)、雌二醇(E₂)及孕酮(PROG)的含量;免疫组化法检测下丘脑组织促性腺激素释放激素(GnRH)、垂体组织促性腺激素释放激素受体(GnRHR)的表达;以蛋白免疫印迹技术检测卵巢组织促卵泡生成素受体(FSHR)、黄体生成素受体(LHR)蛋白表达量。以实时荧光定量PCR检测卵巢组织中FSHR、LHR基因表达量。结果:与空白组比较,乌力吉-18低剂量组可明显升高血清LH含量(P<0.05),上调下丘脑组织GnRH、垂体组织GnRHR表达及卵巢组织FSHR、LHR蛋白表达(P<0.05);乌力吉-18高剂量组可显著升高血清FSH、LH、E₂含量(P<0.05),上调下丘脑组织GnRH表达及卵巢组织FSHR表达量(P<0.05),并可显著升高卵巢组织中FSHR、LHR基因表达量(P<0.05);对照组可明显升高血清E₂含量(P<0.05)。结论:蒙药乌力吉-18可明显升高血清FSH、LH及E₂的含量,促进下丘脑组织GnRH、垂体组织GnRHR及卵巢组织中FSHR、LHR的表达,表明乌力吉-18能够对下丘脑-垂体-卵巢轴相关激素及受体表达产生影响。

Effects of Mongolian Medicine Wuliji-18 on Hypothalamus-Pituitary-Ovarian Axis Related Hormones and Receptors Expression in Rats

Objective:To investigate the effects of Mongolian medicine Wuliji-18 on hypothalamus-pituitary-ovarian axis related hormones and receptors in rats.Methods:40 healthy female nonpregnant SD rats were selected,they were randomly divided into blank group,control group and Wuliji-18 high and low dose groups,with 10 rats in each group.The blank group was given equal volume of distilled water by gavage,the control group was given Xiaoyao pill by gavage,and the high and low dose groups were gavaged with 2.0 g·kg^-1·d^-1 and 1.0 g·kg^-1·d^-1 Wuliji-18 respectively,continuous administration for 31 days.The serum gonadotropin releasing hormone(GnRH),follicle stimulating hormone(FSH),luteinizinghormone(LH),estradiol(E₂)and progesterone(PROG)contents were determined by enzyme-linked immunosorbent assay.The expression of gonadotropin releasing hormone(GnRH)in hypothalamus tissue and gonadotropin releasing hormone receptor(GnRHR)in pituitary tissue were detected by immunohistochemical method.The expression of follicle-stimulating hormone receptor(FSHR)and luteinizing hormone receptor(LHR)in ovarian tissue were detected by western blot.The expressions of FSHR and LHR genes in ovarian tissue were detected by real-time quantitative PCR.Results:Compared with the blank group,Wuliji-18 low dose group could significantly increase the serum LH content(P<0.05),up-regulate the expressions of GnRH in hypothalamus tissue and GnRHR in pituitary tissue,and the protein expressions of FSHR and LHR in ovarian tissue(P<0.05).Wuliji-18 high dose group could significantly increase the serum FSH,LH and E₂ content(P<0.05),up-regulate the expression of GnRH in hypothalamus tissue and FSHR in ovarian tissue(P<0.05),and significantly increase the expression of FSHR and LHR in ovarian tissue(P<0.05).The serum E₂ content in control group was significantly increased(P<0.05).Conclusion:Mongolian medicine Wuliji-18 can significantly increase the serum FSH,LH and E₂ contents,and promote the expression of GnRH in hypothalamic tissue,GnRHR in pituitary tissue and FSHR and LHR in ovarian tissue,thus indicating that Wuliji-18 can affect the expression of hormones and receptors related to the hypothalamic-pituitary-ovarian axis.