HL-1细胞培养及房颤细胞模型的建立

目的:利用HL-1细胞建立快速起搏模型,对心房颤动(atrial fibrillation,AF)早期的重构现象进行初步研究。方法:培养HL-1细胞,建立快速电场刺激起搏细胞模型,利用全细胞膜片钳技术记录刺激前后HL-1细胞的动作电位周期,透射电镜观察细胞超微结构的变化。结果:将细胞接种于培养皿中,72 h后细胞呈融合状态,全细胞膜片钳记录培养HL-1细胞及经电场刺激(600次/min,1 V/cm)24 h后的心房肌细胞的动作电位周期,动作电位周期分别为106 ms,45 ms,刺激前后差异有统计学意义(P0.05)。透射电镜观察到刺激后HL-1细胞超微结构发生去分化改变。结论:经快速起搏24 h后,HL-1细胞发生了电及结构重构;利用HL-1细胞建立快速起搏的房颤模型,可以对房颤早期的重构机制进行研究。

Culture of HL-1 Cardiomyocytes and Establishment of Rapid Paced Cells Model

Objective:To explore early electrical and structural remodeling in atrial fibrillation utilizing a rapid paced cultured HL-1 cardiomyocytes model.Methods:HL-1 cardiomyocytes were cultured and established as a rapid paced cultured HL-1 cardiomyocytes model. HL-1 cells were cultured in the presence of rapid field stimulation (600 beats per min,1 V/cm)for 24 h. Action potential duration was recorded fromstimulated cells and control cells using whole-cell voltage-clamp techniques. Ultrastructure of HL-1 cells was observed under electron microscope.Results:After 72h of culture, the cells were in the confluent phase. The action potential duration was shortened through the rapid pacing compared with those before pacing (P<0.05). Results of electron microscope showed that HL-1cells generated dedifferentiation through pacing.Conclusion:Rapid stimulation of HL-1 cells in culture produces electrical remodeling, recapitulating principal phenotypic features of atrial tachycardia remodeling in vivo. It is available that a rapid paced cell model is established with cultured HL-1 cells to study the mechanismof early electrical and structural remodeling for atrial fibrillation.