人参皂甙Rg3对糖尿病肾病大鼠生化指标及病理的影响

目的:探讨人参皂苷Rg3对糖尿病肾病大鼠生化指标及病理改变的影响。方法:30只SD雄性大鼠按随机数字表法分为正常对照组、模型对照组和人参皂甙Rg3组。采用链脲佐菌素建立糖尿病肾病大鼠模型。造模成功后,Rg3治疗组每天以Rg3(0.5mg/kg)灌胃,余予以等量蒸馏水灌胃。30天后分别测3组大鼠血糖、24小时尿蛋白、血肌酐,并予以HE染色行肾组织活检。结果:与正常组比较,模型对照组大鼠血糖、24小时尿蛋白、血肌酐明显升高,肾小球体积增大,基底膜增厚、细膜基质增多,肾小球内炎细胞浸润(P0.01)。与模型对照组比较,人参皂甙Rg3组血糖、24小时尿蛋白、血肌酐明显降低,肾小球基底膜增厚程度减轻,细胞外基质堆积减少,差异具有显著性(P0.05)。结论:人参皂甙Rg3能显著降低糖尿病大鼠血糖、血肌酐、24 h尿蛋白,能改善其肾脏的病理损害。

The Effect of Ginsenoside Rg3 on the Biochemical Indicators and Pathological of Diabetic Nephropathy Rats

Objective:To investigate the effect of the ginsenoside Rg3 on the biochemical indicators and pathological of diabetic nephropathy rats.Methods:Thirty Sprague-Dawley male rats were randomly divided into 3 groups: normal control group, model control group and Rg3 treated group. The diabetic nephropathy rat model was induced by injection of streptozotocin (STZ, 65 mg/kg) in abdominal cavity. After establishing the model, the Rg3 treated group was daily administrated with Rg3 (0.5 mg/kg) for 30 days, while the other two groups were given distilled water. The blood glucose, twenty-four hours urine protein and serum creatine were measured the day before the rats were killed when the 30 days of treatments had been completed. The renal pathological changes were evaluated by HE staining.Results:The blood glucose, twenty-four hours urine protein and serum creatine increased significantly in the rats of model control group. The volume of renal glomerulus enlarged. The basement membrane was thickened. The cell matrix was increased, as well as some inflammatory cells were accumulated in renal glomerulus (P<0.01). Compared with that in the model control group, the level of blood glucose, twenty-four hours urine protein and serum creatine were deduced obviously in Rg3 treated group. The extent of the thickened basement membrane and the accumulation of extracellular matix reduced (P<0.05).Conclusion:The Rg3 can reduce the level of the blood glucose, twenty-four hours urine protein, serumcreatine and the pathological damage of renal with diabetic nephropathy.