| EDU 免疫荧光法检测整合素连接激酶（ILK）高表达
对新生大鼠心肌细胞DNA 合成的影响 |
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| 引用本文： | 王丙剑,白剑,孙静娴,张娜,徐标.EDU 免疫荧光法检测整合素连接激酶（ILK）高表达
对新生大鼠心肌细胞DNA 合成的影响[J].现代生物医学进展,2011,11(10):1858-1860. |
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| 作者姓名： | 王丙剑 白剑 孙静娴 张娜 徐标 |
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| 作者单位： | 1. 南京医科大学附属鼓楼临床医学院心脏科,江苏,南京,210008
2. 南京大学医学院附属鼓楼医院心脏科,江苏,南京,210008 |
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| 摘 要： | 目的:通过EDU免疫荧光法观察整合素连接激酶(Integrin-Linked Kinase,ILK)在新生大鼠心肌细胞内高表达后对心肌细胞DNA合成的影响。方法:取新生(1-3天内)大鼠原代心肌细胞,培养72小时后随机分为正常对照组、ILK转染组。对照组转染重组腺病毒载体(adeno-GFP),ILK组转染重组腺病毒载体+ILK基因(adeno-ILK)。转然成功后48小时将两组心肌细胞分别通过5-乙基-2’-脱氧尿嘧啶核苷(EDU)免疫荧光法测定心肌细胞DNA合成。结果:ILK转染组心肌细胞内DNA合成较对照组明显增加(P<0.05)。结论:ILK高表达具有促进新生大鼠心肌细胞的DNA合成的能力。
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| 关 键 词： | 整合素连接激酶 心肌细胞 增殖 DNA合成 EDU |
Detection of Over-Expressed Integrin-Linked Kinase in DNA Synthesis of
Cardiomyocytes in Newborn Rats by EDU Immunofluorescence Assay |
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| WANG Bing-jian,BAI Jian,SUN Jing-xian,ZHANG N,XU Biao.Detection of Over-Expressed Integrin-Linked Kinase in DNA Synthesis of
Cardiomyocytes in Newborn Rats by EDU Immunofluorescence Assay[J].Progress in Modern Biomedicine,2011,11(10):1858-1860. |
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| Authors: | WANG Bing-jian BAI Jian SUN Jing-xian ZHANG N XU Biao |
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| Institution: | WANG Bing-jian1,BAI Jian2,SUN Jing-xian1,ZHANG Na2,XU Biao1(1 Department of Cardiology,Drum Tower Clinical Medical College,Nanjing Medical University,210008,Jiangsu,China,2 Department of Cardiology,Nanjing Drum Tower Hospital,Nanjing University Medical School,China) |
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| Abstract: | Objective:To investigate the effects of over-expressed integrin-linked kinase on rat neonatal DNA synthesis of cardiomyocytes by EDU immunofluorescence assay.Methods:Primary neonatal rat(1-3 days old) cardiomyocytes were cultured in vitro and randomly divided into two groups 72 h after isolation:infected by either adeno-GFP(control group) or adeno-GFP containing adeno-ILK(ILK group).48h after infection,DNA synthesis of cardiomyocytes in two groups were determined by 5-ethynyl-2-deoxyuridine(EdU) immunofluor... |
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| Keywords: | Integrin-linked kinase Cardiomyocyte proliferation DNA synthesis EDU |
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