Kv1.3 钾离子通道在卵巢癌细胞株中的表达及活性

目的:研究Kv1.3钾离子通道在SKOV3卵巢癌细胞中的表达及其在细胞增殖和细胞周期中的作用。方法:应用RT-PCR和免疫细胞化学鉴别Kv1.3钾离子通道在SKOV3卵巢癌细胞中的表达。应用MTT和流式细胞技术观察KV1.3钾离子通道对SKOV3卵巢癌细胞增殖及细胞周期的影响。结果:4-氨基吡啶是Kv1.3钾离子通道特异性阻滞剂。不同浓度的4-氨基吡啶可以明显抑制SKOV3细胞的增殖,并且细胞周期也受到影响。G0/G1细胞比例增加,S期和G2/M期细胞比例下降。结论:Kv1.3钾离子通道在SKOV3卵巢癌细胞中表达,并且在细胞增殖及细胞周期变换中扮演着重要的角色。

Expression and Activity of Kv1.3 Potassium Ion Channels in Human Ovarian Cancer Cells

Objective: To investigate the expression of Kv1.3 potassium ion channels in SKOV3 ovarian cancer cell line and the role in cell proliferation and cell cycle. Methods: RT-PCR and immunocytochemical methods were used to identify the expression of Kv1.3 potassium ion channels in SKOV3 cells. The effects of Kv1.3 potassium ion channels on SKOV3 cells proliferation and cell cycle were detected by MTT and flow cytometry. Results: 4-Aminopyridine was an effective blocker of Kv1.3 potassium ion channels. Different concentrations of 4-aminopyridine inhibited the proliferation of SKOV3 cell and affected the cell cycle in a dose-dependent manner, which increased the percentage of cells in G0/G1, and reduced the percentage of cells in S phase and G/M phase. Conclusions: There was expression of the Kv1.3 potassium ion channels in SKOV3 cells and Kv1.3 play an important role in cell proliferation and cell cycle.