| 乙型肝炎病毒核心抗体IgM的蛋白芯片检测法研究 |
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| 引用本文： | 吴 敏,陈俊梅,刘召波,刘海东,刘 超,张爱英,李 宁.乙型肝炎病毒核心抗体IgM的蛋白芯片检测法研究[J].现代生物医学进展,2019,19(3):560-562. |
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| 作者姓名： | 吴 敏 陈俊梅 刘召波 刘海东 刘 超 张爱英 李 宁 |
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| 作者单位： | 首都医科大学附属北京佑安医院;北京市肝病研究所; |
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| 基金项目： | 北京市科技委员会基金项目(Z181100001018031) |
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| 摘 要： | 目的:探索蛋白芯片技术检测乙型肝炎病毒(HBV)抗HBc-IgM的可行性。方法:应用Nano-Plotter TM-压电式微量喷墨点阵制备系统自制的核心抗原蛋白芯片在经10%山羊血清封闭后加入待测血清37℃孵育,PBST清洗晾干后后再次加入检测抗体即HRP-抗人Ig M抗体,芯片用伯乐成像仪检测有无信号。结果:24份HBs Ag和抗HBc阳性血清中经蛋白芯片检测抗HBc-IgM阳性率为83.3%(20/24);24份健康志愿者血清中经蛋白芯片检测抗HBc-IgM阳性率为4.1%(1/24)。乙肝血清与健康志愿者血清化学发光信号差异明显。结论:蛋白芯片技术可较好地应用于定性检测HBV抗HBc-IgM,为临床快速判断是否存在HBV感染和监测慢性肝炎HBV活动性提供新的辅助诊断方法。
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| 关 键 词： | 蛋白芯片 乙肝病毒 定性 抗HBc-IgM |
| 收稿时间： | 2018/5/22 0:00:00 |
| 修稿时间： | 2018/6/18 0:00:00 |
Detection of Human Anti-hepatitis B Virus Core Antibody IgM by Protein Microarray |
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| WU Min,CHEN Jun-mei,LIU Zhao-bo,LIU Hai-dong,LIU Chao,ZHANG Ai-ying and LI Ning.Detection of Human Anti-hepatitis B Virus Core Antibody IgM by Protein Microarray[J].Progress in Modern Biomedicine,2019,19(3):560-562. |
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| Authors: | WU Min CHEN Jun-mei LIU Zhao-bo LIU Hai-dong LIU Chao ZHANG Ai-ying and LI Ning |
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| Institution: | Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China,Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China,Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China,Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China,Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China,Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China and 1 Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China;2 Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing, 100069, China |
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| Abstract: | |
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| Keywords: | Protein Microarray Hepatitis B virus Qualitative Anti-HBc IgM |
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