| 三维联合培养牙髓细胞和血管内皮祖细胞促进成牙本质向/成骨向分化 |
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| 引用本文： | 陈 婷,侯 晋,李心竹,宋 词,张雪洋,吴补领.三维联合培养牙髓细胞和血管内皮祖细胞促进成牙本质向/成骨向分化[J].现代生物医学进展,2019,19(16):3028-3032. |
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| 作者姓名： | 陈 婷 侯 晋 李心竹 宋 词 张雪洋 吴补领 |
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| 作者单位： | 南方医科大学南方医院口腔科;南方医科大学顺德医院(佛山市顺德区第一人民医院);南方医科大学口腔医学院 |
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| 基金项目： | 国家自然科学基金项目（81800950；81870755；81670986） |
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| 摘 要： | 目的:探讨三维联合培养牙髓细胞(dental pulp cells, DPCs)和血管内皮祖细胞(endothelial progenitor cells, EPCs)对成牙本质向/成骨向分化的影响。方法:取单独培养DPCs及联合培养的DPCs和EPCs进行三维培养后成牙本质向/成骨向诱导,使用茜素红染色及半定量分析、RT-PCR和细胞免疫荧光检测成牙本质向/成骨向分化能力。采用SPSS 23.0统计软件对数据进行统计学分析。结果:茜素红染色显示联合培养组和单独培养组之间未见显著差异。RT-PCR和细胞免疫荧光显示成牙本质向/成骨向相关基因m RNAs和蛋白表达水平联合培养组显著高于单独培养组。结论:三维联合培养的DPCs和EPCs促进成牙本质向/成骨向分化,为牙髓再生提供可能实验依据。
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| 关 键 词： | 牙髓细胞 血管内皮祖细胞 三维联合培养 成牙本质向/成骨向分化 |
| 收稿时间： | 2019/5/6 0:00:00 |
| 修稿时间： | 2019/5/30 0:00:00 |
Three-dimensional Coculture of Dental Pulp Cells and Endothelial Progenitor Cells Enhances Osteo/odontogenic Differentiation |
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| CHEN Ting,HOU Jin,LI Xin-zhu,SONG Ci,ZHANG Xue-yang,WU Bu-ling.Three-dimensional Coculture of Dental Pulp Cells and Endothelial Progenitor Cells Enhances Osteo/odontogenic Differentiation[J].Progress in Modern Biomedicine,2019,19(16):3028-3032. |
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| Authors: | CHEN Ting HOU Jin LI Xin-zhu SONG Ci ZHANG Xue-yang WU Bu-ling |
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| Institution: | 1 Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China; 2 Shunde Hospital, Southern Medical University (The First People''s Hospital of Shunde Foshan), Foshan, Guangdong, 528300, China; 3 School of Stomatology, Southern Medical University, Guangzhou, Guangdong, 510515, China;Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China; School of Stomatology, Southern Medical University, Guangzhou, Guangdong, 510515, China |
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| Abstract: | ABSTRACT Objective: To observe the effect of three-dimensional coculture of dental pulp cells (DPCs) and endothelial progenitor cells (EPCs) on the osteo/odontogenic differentiation. Methods: After osteo/odontogenic induced of three-dimensional culture of the coculture of DPCs and EPCs and the culture of DPCs, Alizarin red stain, RT-PCR and immunofluorescence were used to detect the osteo/odontogenic differentiation. The data was analyzed using SPSS 23.0 software package. Results: Alizarin red stain showed that it is no significant differences between the coculture group and the culture group. RT-PCR and immunofluorescence showed that the coculture group is significant higher than the culture group on the mRNA and protein level of osteo/odontogenic differentiation. Conclusion: Three-dimensional coculture of dental pulp cells and endothelial progenitor cells enhances osteo/odontogenic differentiation. |
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| Keywords: | Dental pulp cells Endothelial progenitor cells Three-dimensional coculture Osteo/odontogenic differentiation |
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