UBE2T基因对结直肠细胞增殖和凋亡的影响

目的:探讨人类泛素结合酶E2T(Ubiquitin-conjugating enzyme E2T,UBE2T)基因对结肠细胞增殖和凋亡的影响。方法:体外培养人正常结直肠粘膜细胞FHC,采用将UBE2T基因慢病毒质粒转染至FHC细胞48 h后,通过MTT法检测细胞增殖情况,western blotting检测细胞中增殖相关蛋白UBE2T蛋白、Ki67、促凋亡蛋白Bax和抗凋亡蛋白Bcl-2的表达,流式细胞术检测细胞凋亡率。结果:与转染空质粒的FHC细胞相比,UBE2T基因慢病毒质粒转染FHC细胞48 h后,细胞增殖能力显著上调(P0.05),UBE2T蛋白明显增加,Ki67的表达明显增加(P0.05),细胞凋亡率显著降低(P0.05),且Bax的表达明显下调而Bcl-2的表达上调(P0.05)。结论:UBE2T基因能够促进正常结肠粘膜细胞的增殖,并抑制其凋亡。

Effect of UBE2T Gene on the Proliferation and Apoptosis of Colorectal Cells

ABSTRACT Objective: To explore the effects of UBE2T(Ubiquitin-conjugating enzyme E2T) gene on the apoptosis of colorectal cells. Methods: Normal colorectal mucosal cells FHC were cultured in vitro and transfected by UBE2T lentivirus plasmid for 48 h, MTT was used to detect the cell proliferation; the UBE2T protein, the proliferation-associated protein Ki67, the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl-2 in cells were detected by Western Blotting; the apoptosis rate of cells was evaluated by Flow cytometry (FCM). Results: Compared with FHC cells transfected with empty plasmid, the proliferation ability of FHC cell transfected by UBE2T lentivirus plasmid was obviously upregulated (P<0.05), the expression of UBE2T protein and Ki67 protein were significantly increased, the apoptosis rate was obviously decreased, the expression of Bax protein was significantly decreased and the expression of Bcl-2 protein was obviously increased(P<0.05). Conclusion: UBE2T gene promoted the proliferation and inhibited the apoptosis of normal colorectal cells FHC.