| 对慢性鼻-鼻窦炎患者细菌生物膜检测方法的改良 |
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| 引用本文： | 李 晗,孙希才,刘 全,李艳青,胡 俐,于华鹏,王德辉.对慢性鼻-鼻窦炎患者细菌生物膜检测方法的改良[J].现代生物医学进展,2019,19(2):217-221. |
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| 作者姓名： | 李 晗 孙希才 刘 全 李艳青 胡 俐 于华鹏 王德辉 |
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| 作者单位： | 复旦大学附属眼耳鼻喉科医院耳鼻咽喉科 |
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| 基金项目： | 国家自然科学基金项目（81600783）；上海市科委基金资助项目（15401971500） |
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| 摘 要： | 目的:应用BacLight/CSLM观察BBF时要求使用无菌超纯水进行标本制备,超纯水的低渗作用使活体细胞明显减少,干扰观察结果,本研究提出使用无菌生理盐水代替超纯水进行标本制备,以改良检测效果。方法:在鼻内镜手术中收取27例慢性鼻-鼻窦炎(CRS)患者的鼻窦黏膜,使用激光共聚焦显微镜(CSLM)检测细菌生物膜(BBF)。对16例BBF阳性病例,分别使用无菌生理盐水、超纯水两种方法进行样本制备,采用BacLight/CSLM方法检测样本中的BBF,分别进行BBF评分,统计分析两组评分的差异。结果:生理盐水组更真实地还原了CRS患者鼻窦黏膜中的BBF图像。超纯水组的BBF评分显著低于生理盐水组,两组评分有显著性差异(2.125±0.885 vs. 1.500±0.632, P=0.029)。结论:生理盐水法检测BBF更真实地还原CRS患者鼻窦中黏膜细胞和BBF的形态,有助于准确进行BBF评分。
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| 关 键 词： | 慢性鼻-鼻窦炎 细菌生物膜 激光共聚焦显微镜 细菌生物膜评分 |
| 收稿时间： | 2018/7/26 0:00:00 |
| 修稿时间： | 2018/8/18 0:00:00 |
Improvement of BacLight/CSLM Criteria for Detection of Bacterial Biofilm in Chronic Rhinosinusitis |
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| LI Han,SUN Xi-cai,LIU Quan,LI Yan-qing,HU Li,YU Hua-peng and WANG De-hui.Improvement of BacLight/CSLM Criteria for Detection of Bacterial Biofilm in Chronic Rhinosinusitis[J].Progress in Modern Biomedicine,2019,19(2):217-221. |
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| Authors: | LI Han SUN Xi-cai LIU Quan LI Yan-qing HU Li YU Hua-peng and WANG De-hui |
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| Institution: | Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China,Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China,Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China,Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China,Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China,Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China and Department of Otolaryngology, Affiliated Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China |
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| Abstract: | ABSTRACT Objective: Presently, BacLight/CSLM criteria require sterile MQ water for the preparation of specimen in bacterial biofilm (BBF) detection. The hypotonic effect of sterile MQ water disturbs observation results by reducing living cells. In this study, the samples were prepared with sterile natural saline(NS) and sterile MQ water, respectively, and differences between two methods were compared. Methods: The mucosa of nasal sinus of 27 patients with chronic rhinosinusitis(CRS) was collected during endoscopic sinus surgery, and BBF was detected by laser confocal microscopy(CSLM). For 16 BBF positive cases, BBF samples were prepared by sterile NS and sterile MQ water respectively. BacLight/CSLM method was applied in the observation of BBF, and BBF scores were measured. Difference of BBF score between the two groups was statistically analyzed. Results: BBF image was restored more accurately in NS group. BBF score of MQ water group was significantly lower than that of NS group (2.125±0.885 vs. 1.500±0.632, P=0.029). Conclusion: De- tection of BBF by NS method reflects the morphology of mucosal cells and BBF in the paranasal sinuses more accurately, and is helpful for BBF scoring. |
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| Keywords: | Chronic rhinosinusitis Bacterial biofilm Confocal laser scanning microscope Bacterial biofilm score |
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