| 耐辐射奇球菌pprM基因增强大肠杆菌氧化抗性的研究 |
| |
| 引用本文： | 阳 志,李 伟,王五洲,贺俊彦,肖方竹,贺 特,曹旭琴,马 云,何淑雅.耐辐射奇球菌pprM基因增强大肠杆菌氧化抗性的研究[J].现代生物医学进展,2019,19(3):429-433. |
| |
| 作者姓名： | 阳 志 李 伟 王五洲 贺俊彦 肖方竹 贺 特 曹旭琴 马 云 何淑雅 |
| |
| 作者单位： | 南华大学公共卫生学院放射医学教研室;中南大学湘雅医院;南华大学生物化学与分子生物学教研室; |
| |
| 基金项目： | 国家自然科学基金项目(81741143)；湖南省教育厅科研项目(17A186；2018JJ3458) |
| |
| 摘 要： | 目的:探讨耐辐射奇球菌ppr M基因对大肠杆菌氧化抗性的影响。方法:氯化钙法转化分别构建含pGEX-6p-1-pprM质粒的大肠杆菌DH5α。测定不同浓度过氧化氢对含pGEX-6p-1-pprM、pGEX-6p-1和野生型大肠杆菌DH5α活性的影响以及菌体内SOD/GSH/CAT水平的变化。结果:与空质粒组和野生型组相比,含pprM的大肠杆菌在同浓度过氧化性情况下,其抑菌圈明显缩小,差异有统计学意义。与空质粒组和野生型组相比,含pprM的大肠杆菌体内CAT活力、SOD活性明显提高,但GSH量并没有明显提高。结论:pprM基因能够提高大肠杆菌抗氧化能力,其机制可能与pprM基因增强细菌体内抗氧化酶的活性有关。
|
| 关 键 词： | 耐辐射奇球菌 大肠杆菌 pprM 过氧化氢 抗氧化 |
| 收稿时间： | 2018/7/21 0:00:00 |
| 修稿时间： | 2018/8/16 0:00:00 |
Enhances of the Antioxidant Capacity in Escherichia coli by Deinococcus radiodurans pprM |
| |
| YANG Zhi,LI Wei,WANG Wu-zhou,HE Jun-yan,XIAO Fang-zhu,HE Te,CAO Xu-qin,MA Yun and HE Shu-ya.Enhances of the Antioxidant Capacity in Escherichia coli by Deinococcus radiodurans pprM[J].Progress in Modern Biomedicine,2019,19(3):429-433. |
| |
| Authors: | YANG Zhi LI Wei WANG Wu-zhou HE Jun-yan XIAO Fang-zhu HE Te CAO Xu-qin MA Yun and HE Shu-ya |
| |
| Institution: | Department of radiology medicine, School of public health, University of South China, Hengyang, Hunan, 421001, China,Xiangya Hospital of Central South University, Hengyang, Hunan, 410008, China,Department of biochemistry and molecular biology, School of pharmacy and biological Sciences, University of South China, Hengyang, Hunan, 421001, China,Department of radiology medicine, School of public health, University of South China, Hengyang, Hunan, 421001, China,Department of radiology medicine, School of public health, University of South China, Hengyang, Hunan, 421001, China,Department of biochemistry and molecular biology, School of pharmacy and biological Sciences, University of South China, Hengyang, Hunan, 421001, China,Department of radiology medicine, School of public health, University of South China, Hengyang, Hunan, 421001, China,Department of biochemistry and molecular biology, School of pharmacy and biological Sciences, University of South China, Hengyang, Hunan, 421001, China and 1 Department of radiology medicine, School of public health, University of South China, Hengyang, Hunan, 421001, China;2 Department of biochemistry and molecular biology, School of pharmacy and biological Sciences, University of South China, Hengyang, Hunan, 421001, China |
| |
| Abstract: | |
| |
| Keywords: | Deinococcus radiodurans Escherichia coli pprM Hydrogen peroxide Antioxidation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《现代生物医学进展》浏览原始摘要信息 |
| 点击此处可从《现代生物医学进展》下载免费的PDF全文 |
|
