Ⅰ期非小细胞肺癌组织血管内皮生长因子C、细胞角蛋白19表达与患者预后的相关性

目的:分析Ⅰ期非小细胞肺癌(NSCLC)组织血管内皮生长因子C(VEGF-C)、细胞角蛋白19(CK19)水平的变化,并探讨其与患者预后的相关性。方法:采用免疫组化检测肺癌组织中VEGF-C、CK19蛋白的表达,分析其与肺癌患者临床病理特点的相关性。随访至少3年,采用Kaplan-Meier法分析不同VEGF-C、CK19蛋白表达患者术后复发及疾病进展时间的差异。结果:VEGF-C和CK19在不同分化程度、胸膜侵犯患者肺癌组织中的表达比较差异均有统计学意义(P0.05)。VEGF-C表达与CK19表达呈显著正相关(r=0.483,P0.05)。术后3年,VEGF-C和CK19阳性患者复发率均显著高于VEGF-C和CK19阴性患者(x~2=14.16, 17.25,P0.05)。Kaplan-Meier法显示VEGF-C阳性、阴性者中位TTP分别为29.2个月(95%CI:27.5～30.9)、36.2个月(95%CI:35.0～37.5);CK19阳性、阴性者中位TTP分别为28.4个月(95%CI:26.6～30.2)、37.8个月(95%CI:36.4～39.1)。VEGF-C、CK19阴性者与阳性者的中位TTP比较差异有统计学意义(x~2=15.77、18.45,P0.05)。结论:I期NSCLC患者肺癌组织VEGF-C、CK19呈高表达,与术后局部复发密切相关,并可作为患者预后评估的参考指标。

Correlation of the Level of Vascular Endothelial Growth Factor C, Cytokeratin 19 with the Prognosis of Patients with Non-small Cell lung Cancer of Stage I

ABSTRACT Objective: To analyze the changes in the level of vascular endothelial growth factor C(VEGF-C), cytokeratin 19(CK19) in patients with non-small cell lung cancer (NSCLC) of stage I, and investigate its correlation with the prognosis of postoperative patients. Methods: Immunohistochemistry was used to detect the expression level of VEGF-C, CK19 protein in lung cancer tissue, and its correlation with the clinicopathological characteristics of lung cancer patients was analyzed. All the patients were followed for at least 3 years, Kaplan-Meier was used to analyze the differecnce of postoperative recurrence and time to progress (TTP) between different levels of VEGF-C, CK19 protein expression. Results: There were significant differences in the expression of VEGF-C and CK19 between the lung cancer tissues with different degrees of differentiation and pleural invasion(P<0.05). The expression of VEGF-C and CK19 showed a significant positive correlation (r=0.483, P<0.05). Three years after surgery, the recurrence rates of patients with VEGF-C and CK19 positive expression were significantly higher than those of patients with VEGF-C and CK19 negative expression(x=14.16, 17.25, P<0.05). Kaplan-Meier survival analysis showed that the median TTP in positive and negative VEGF-C expression group were 29.2 months(95%CI: 27.5~30.9), 36.2 months (95%CI:35.0~37.5). The median TTP in positive and negative CK19 expression group were 28.4 months(95%CI: 26.6~30.2), 37.8 months (95%CI:36.4~39.1). There were significantly difference on the median TTP between positive and negative VEGF-C and CK19 expression (P<0.05). Conclusion: The expression of VEGF-C and CK19 in the lung cancer tissue of NSCLC patients of stage I were upregulated and closely related to the postoperative local recurrence, it can be used as an reference index for the prognostic evaluation.