| 泛素连接酶gp78促进体外肝细胞脂肪变性的作用研究 |
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| 引用本文： | 李洁,张丰,刘芳,张志文,徐玉乔,李青.泛素连接酶gp78促进体外肝细胞脂肪变性的作用研究[J].现代生物医学进展,2016,16(4):665-668. |
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| 作者姓名： | 李洁 张丰 刘芳 张志文 徐玉乔 李青 |
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| 作者单位： | 第四军医大学基础部病理学教研室 |
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| 基金项目： | 国家自然科学基金项目(81170798);国家自然科学基金青年基金项目(31400722) |
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| 摘 要： | 目的:研究泛素连接酶gp78在体外肝细胞脂肪变性发生过程中的作用。方法:在小鼠肝细胞内转染携带gp78的质粒DNA,400μM油酸刺激肝细胞,油红O染色观察细胞内脂滴形成的情况;测定肝细胞内甘油三酯含量;western blot和RT-PCR检测细胞内gp78的表达水平。结果:与对照组相比,过表达gp78肝细胞内脂滴数目增多,体积增大,甘油三酯含量(4.6±1.56)mol/L升高;而沉默gp78肝细胞内脂滴数目减少,体积减小,甘油三酯含量(0.3±1.37)mol/L明显降低(P0.05)。结论:在肝细胞发生脂肪变性过程中,gp78可能发挥了重要的作用;而其发挥作用的机制还有待进一步研究。
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| 关 键 词： | gp78 肝细胞 脂肪变性 |
The Role of gp78 in Promoting the Hepatic Steatosis in Vitro |
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| LI Jie;ZHANG Feng;LIU Fang;ZHANG Zhi-wen;XU Yu-qiao;LI Qing.The Role of gp78 in Promoting the Hepatic Steatosis in Vitro[J].Progress in Modern Biomedicine,2016,16(4):665-668. |
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| Authors: | LI Jie;ZHANG Feng;LIU Fang;ZHANG Zhi-wen;XU Yu-qiao;LI Qing |
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| Institution: | LI Jie;ZHANG Feng;LIU Fang;ZHANG Zhi-wen;XU Yu-qiao;LI Qing;Depment of Pathology, Basic Medical College, the Fourth Military Medical University; |
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| Abstract: | Objective:To study the role of gp78 in the process of fatty degeneration of liver cells in vitro.Methods:Using oil red
O staining to observe the formation of lipid droplets in mouse liver cells, determine triglyceride in liver cells. Expression levels of gp78 in
liver cells were detected by Western blot and RT-PCR.Results:As compared to the control group, Overexpression of gp78 could increase
the number of lipid droplets and upregulate triglyceride levels. While depletion of gp78 could decrease lipid droplets numbers, and also
could downregulate the content of triglyceride.Conclusion:Our results strongly suggests that gp78 might play an important role during
the course of hepatic steatosis. |
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| Keywords: | gp78 Hepatic cell Steatosis |
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