乙肝免疫标志物与乙肝病毒核酸含量与肝功能的关系研究

目的:研究乙肝免疫标记物(HBV-M)与乙肝病毒脱氧核苷酸(HBV-DNA)载量与肝功能的关系。方法:检测104例乙肝患者HBs Ag、HBe Ag、HBc Ab、HBs Ab、HBe Ab5种HBV-M;用PCR法检测HBV-DNA含量;同时检测天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、胆碱酯酶(CHE)等肝功能指标,对三者关系采用Spearman相关性分析。结果:HBs Ag、HBc Ab、HBe Ag阳性组HBV-DNA阳性率及载量均大于其余组(P0.05);HBe Ag含量、ALT浓度与HBV-DNA载量均呈正相关(r=0.48,P0.05)、(r=0.36,P0.05);AST、CHE与HBV-DNA含量无明显相关性,但在无病毒载量与有病毒载量组之间AST比较有统计学意义(P0.05)。结论:HBV-M、HBV-DNA与肝功能之间有一定的相关性,但HBe Ag阴转不代表病毒复制停止,HBV-DNA也不能完全反应肝损害程度,临床应加大重视。

Relationship of Hepatitis B Immune Markers and HBV-DNA Content with Liver Function

Objective:To study the relationship of HBV-M and HBV-DNA content with liver function.Methods:Detected the HBsAg, HBeAg, HBcAb, HBsAb and HBeAb levels of 104 hepatitis B patients. PCR method was used to detect their HBV-DNA levels. Meanwhile, their aspartate aminotransferase(AST), alanine aminotransferase enzymes(ALT), cholinesterase(CHE) levels were detected, and their relations between each other were obtained by using Spearman correlation analysis.Results:The positive rate of HBV-DNA and its load volume was greater in HBsAg, HBcAb, HBeAg-positive group than in the other groups(P<0.05). The HBeAg levels and ALT concentration was positively correlated with HBV-DNA load (r=0.48, P<0.05), (r=0.36, P<0.05). AST and CHE showed no significant correlation with HBV-DNA load, but AST between no viral group and viral group showed statistical difference (P<0.05)Conclusion:There is a certain correlation between HBV-M, HBV-DNA and liver function. But HBeAg seroconversion does not mean to stop viral replication, and yet HBV-DNA can not completely reflect liver damage. So we should pay more attention in clinic.