跑台训练与强制性运动对脑梗死大鼠神经功能恢复的对比性研究

目的:探讨跑台训练与强制性运动康复训练对脑梗死大鼠神经功能恢复的差异。方法:将50只SD雄性大鼠随机分为正常组、假手术组、模型组、跑台训练组(T组)、强制性运动训练组(C组),每组10只,T组及C组于造模后24 h分别进行跑台训练及强制性运动训练,其他各组正常饲养。于术后14天、21天及28天分别进行神经功能缺损评分(NSS评分),随后取脑采用免疫组织化学方法观察脑梗死灶周围巢蛋白(Nestin)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)、神经胶质酸性蛋白(GFAP)的表达。结果:各亚组同一时间NSS评分组间比较:C组、T组评分高于正常组及假手术组,但明显低于模型组(P0.05);C组评分明显低于T组(P0.05);各组内比较差异无统计学意义(P0.05)。C组、T组Caspase-3的表达明显低于正常组、假手术组及模型组(P0.05),而Nestin、GFAP的表达高于正常组、假手术组及模型组(P0.05)。T组Caspase-3的表达明显高于C组(P0.05),Nestin、GFAP的表达低于C组(P0.05)。结论:跑台训练与强制性运动康复训练2种方法对脑梗死大鼠神经功能恢复均有效;强制性运动的效果优于跑台训练。

Comparative Study of Treadmill Training and Constraint-induced Movement Therapy on the Recovery of Neural Function in Rats with Cerebral Infarction

Objective:To investigate the difference between treadmill training and constraint-induced movement therapy on the neural function after middle cerebral artery occlusion in rats.Methods:50 healthy female SD rats were randomly divided into 5 groups: normal group, sham operation group, model group, treadmill training group (T group) and constraint-induced movement therapy group(C group), with 1 0 rats in each group. Treadmill training and constraint-induced movement therapy were used respectively for T group and C group after modeling, while rats in the other groups were given normal feeding. The neurologic impairment scores(NSS) were analyzed at 14 days, 21 days and 28 days respectively after operation. And immuno-histochemical technique was used to observe the expression levels of Nestin, Caspase-3 and GFAP in cortical ischaemic lesions of the rats in each group after 28 days.Results:Comparison of each sub group at the same time: the scores of NSS in C group and T group were significantly higher than that in normal group and sham operation group, but lower than that in the model group (P<0.05); the scores of NSS in C group were significantly lower than that in the T group (P<0.05). There was no significant difference at the different time points in the same group (P>0.05). The expression levels of Caspase-3 in C group and T group were significantly lower than that in normal group, sham operation group and model group(P<0.05), while the expression levels of Nestin and GFAP were just the opposite(P<0.05); the expression levels of Caspase-3 in T group significantly were lower than that in C group (P<0.05), but the expression levels of Nestin and GFAP were just the opposite (P<0.05).Conclusion:The two methods of treadmill training and constraint-induced movement therapy were effective on neural functional recovery in rats with cerebral infarction; constraint-induced movement therapy was better than treadmill training.