趋化因子CCL-18 在慢性鼻- 鼻窦炎中的表达及意义

目的:分析趋化因子CCL-18在不同组织病理特征慢性鼻-鼻窦炎和正常鼻黏膜的表达差异,探讨CCL-18在慢性鼻-鼻窦炎中的表达及意义。方法:采用苏木精-伊红染色(HE),Masson染色及过碘酸-雪夫(PAS)染色对慢性鼻-鼻窦炎组织进行病理分析。采用Western blot检测CCL-18蛋白水平在不同组织病理特征慢性鼻-鼻窦炎和正常鼻黏膜组织中的表达差异。结果:CCL-18蛋白水平在伴鼻息肉和不伴有鼻息肉慢性鼻窦炎均较正常鼻黏膜组织中显著上调(P<0.05)。CCL-18蛋白水平在嗜酸性粒细胞慢性鼻窦炎的表达水平明显高于非嗜酸性粒细胞慢性鼻窦炎(P<0.05)。腺体型,纤维炎症型及水肿型慢性鼻-鼻窦炎中CCL-18表达水平均高于正常鼻黏膜,以水肿型表达最为显著(P<0.05)。结论:CCL-18在嗜酸性粒细胞和水肿型慢性鼻窦炎中高度表达,提示CCL-18可能参与慢性鼻-鼻窦炎中嗜酸性粒细胞的浸润这一基本病理过程。

Expression and Significance of Chemokines CCL-18 in Chronic Rhinosinusitis

Objective:To analyze the differential expression of chemokines CCL-18 in various pathological features of the chronic rhinosinusitis and normal nasal mucosa tissues, and to explore the mechanism and significance of CCL-18 in the chronic rhinosinusitis.Methods:Hematoxylin-eosin (HE), Masson trichrome and periodic acid-Schiff (PAS) staining of the chronic rhinosinusitis tissues were used for pathological analysis. Western blot was adopted to detect the expression of CCL-18 protein level in nasal tissues from different pathological features of the chronic rhinosinusitis and in normal nasal mucosa.Results:The expression of CCL-18 protein level was significantly increased in the chronic rhinosinusitis with nasal polyps (CRSwNP), chronic rhinosinusitis without nasal polyps (CRSsNP) compared with normal nasal mucosa (P <0.05). The expression of CCL-18 protein levels was significantly higher in the eosinophils chronic rhinosinusitis tissues than in the non-eosinophils chronic sinusitis tissues (P <0.05). The expression of CCL-18 protein level was higher in the glandular type, fiber inflammation and edema histopathological type of the chronic rhinosinusitis than in normal nasal mucosa tissues. And the most significant expression of CCL18 was present in edema histopathological type (P<0.05).Conclusion:CCL-18 is highly expressed in eosinophils and edema histopathological type of the chronic rhinosinusitis, suggesting that CCL-18 may be involved in the basic pathological process of eosinophil infiltration in the chronic rhinosinusitis.