麦冬皂苷D对过氧化氢造模的HUVEC保护作用机制研究

目的:研究麦冬皂苷D对HUVEC凋亡相关分子的影响,探讨其作用机制。方法:用H2O2构建凋亡模型,运用MTT及流式细胞仪检测细胞活性,激光共聚焦方法测定粒体膜电位和钙离子浓度。结果:麦冬皂苷D可以稳定线粒体膜电位,减少钙离子内流,增加细胞的活力。结论:麦冬皂苷D对HUVEC有一定的保护作用。

Research on the mechanism of ophiopogonin D protecting HUVEC induced by H2O2

Objectives:To investigate the effect of ophiopogonin D on the apoptosis of vascular cell inducted by H2O2, and to re-veal the molecular mechanism.Method:Apoptosis model of HUVEC was established by inducing with H2O2.The activity of HUVEC cell was detected by MTT assay with flow cytometry.Mitochondrial membrane potential and Ca2 concentration in HUVEC was deter-mined by laser scanning confocal microscope.Results:The presence of ophiopogonin D increased the activity of HUVEC cell by stabiliz-ing mitochondrial membrane potential and decreasing intracellular Ca2 .Conclusions:Ophiopogonin D protects the vascular endothelial cell against apoptosis induced by H2O2.