口腔鳞癌细胞系NTCR中侧群细胞的相关研究

目的:口腔鳞癌是口腔颌面部常见的恶性肿瘤之一,本研究以侧群细胞为肿瘤干细胞突破口,通过检测、分选口腔鳞癌细胞系NTCR中侧群细胞(side population,SP)细胞亚群,深入研究不同细胞亚群的体内、外相关生物学特性,寻找口腔鳞癌中肿瘤干细胞存在的证据。方法:选取口腔鳞癌细胞系NTCR作为研究对象,Hoechst 33342染色后行流式细胞仪检测,分选口腔鳞状细胞癌中的SP细胞和非SP细胞,进行体外培养、长期分化和体内成瘤实验,对2种亚群细胞的体内和体外生物学特性进行检测和比较。结果:口腔鳞状细胞癌细胞系NTCR中含有9.3%SP细胞,其SP细胞在细胞的增殖能力、自我更新能力及裸鼠体内成瘤能力等方面与干细胞特性相似。结论:SP细胞可以认为是肿瘤干细胞的富集。进一步深入研究,有可能作为口腔鳞癌诊断、治疗和预后的靶标。

Correlational Research on Side Population Cells of OSCC Cell Line NTCR

Objective:Oral squamous cell carcinoma is one of the most common malignant tumors in the head and neck. To find the existing evidences of cancer stemcells, the side population (SP) cells in oral squamous cell carcinoma cell line NTCR was examined and sorted, and the biological characteristics of in vivo and in vitro were studied.Methods:Hoechst 33342 dye was used to explore and sort SP cells and non-SP cells with the object of studying oral squamous cell carcinoma cell line NTCR. The two subpopulation cells'' proliferation, differentiation and tumorigenicity in nude mice were compared in vivo and in vitro.Results:9.3 % SP cells were found in NTCR cells. And its histological characteristic in proliferation, differentiation and tumorigenicity in nude mice was especially similar with stem cells.Conclusion:SP in oral squamous cell carcinoma is enriched with stem-like cancer cells, and may be an important target for detection, treatment and prognosis of OSCC.