偏头痛发病机制中ASICs通道及P/Q钙通道交互影响的电生理研究

目的:ASICs通道及P/Q钙通道均参与偏头痛发生,分析ASICs通道及P/Q钙通道的电生理相互作用,评价二者的在偏头痛发生中的交互影响。方法:健康SPF级野生型C57BL/6鼠婴,分离培养双侧三叉神经节神经元,采用全细胞膜片钳技术记录三叉神经节神经元的钙电流变化及动作电位变化。结果:酸性外液及阿米洛利对钙通道无直接影响,酸性外液及P/Q通道阻断剂Aga-IVA均增加三叉神经元兴奋性(P0.05),而阿米洛利可阻断这种增加效应(P0.05)。结论:阿米洛利能够抑制Aga-IVA对三叉神经节神经元兴奋性的增加,可能与其阻断ASICs通道有关,提示ASICs通道可能为P/Q通道突变引发偏头痛的下游机制之一。

Electrophysiological Study on Interaction of ASICs Channels and P/Q Calcium Channels in the Pathogenesis of Migraine

ABSTRACT Objective: To evaluate the electrophysiological effects of Acid-sensing cation channels (ASICs) and P/Q-type channel which were all involved in migraine. Methods: Trigeminal ganglion (TG) tissues were collected from healthy SPF wild-type C57BL/6 mice, whole-cell patch clamp technique was used to record the calcium currents of TG neurons and the changes of action potentials. Results: Acid and amiloride had no effect on Calcium channel directly, the acid and Aga-IVA increased the excitability of trigeminal neurons, (P <0.05), and Amiloride blocked this increments (P<0.05). Conclusion: Amiloride can inhibit the increase of excitability of TG neurons in- duced by Aga-IVA, which may be related to the blockage of ASICs channels, indicating that ASICs channels may involve in the mecha- nisms of migraine caused by P/Q channel mutations.