DDX5、E-cadherin在非小细胞肺癌中的表达及其临床意义

目的:探讨DEAD-box家族的DDX5(RNA解旋酶)和E-钙黏蛋白(E-cadherin)在非小细胞肺癌(NSCLC)组织中表达,并分析其临床病理意义。方法:采用免疫组织化学方法(SP法)和Western blot法检测手术切除的NSCLC组织74例及癌旁组织(距肿瘤5 cm)36例中DDX5和E-cadherin的表达情况,并对其与NSCLC患者的临床病理特征的相关性进行统计学分析。结果:NSCLC组织中DDX5的阳性表达率显著高于癌旁组织(63.5%vs 30.6%),E-cadherin的阳性表达率显著低于癌旁组织(60.8%vs 100%),差异均具有统计学意义(P0.05);DDX5和E-cadherin蛋白的表达水平与NSCLC的TNM分期以及淋巴结是否转移具有显著相关性(P0.05),但二者与NSCLC患者的年龄、性别和肿瘤组织类型均无显著相关性(P0.05)。DDX5和E-cadherin蛋白的表达呈显著负相关(r=-0.327,P0.05)。结论:DDX5的过度表达及E-cadherin的表达下调可能参与了NSCLC的发生发展,且二者在其中可能也具有相互作用的关系。

The Expressions and Clinical Significances of DDX5 and E-cadherin in the Non-small Cell Lung Cancer

ABSTRACT Objective: To investigate the expressions and clinical significances of DEAD-box RNA helicase 5(DDX5) and E-cad- herin in the non-small cell lung cancer(NSCLC). Methods: The expressions of DDX5 and E-cadherin were measured with immunohisto- chemistry technique and western blot in 74 cases of non-small cell lung cancer tissues and 36 cases of adjacent normal tissues (5 cm away from carcinoma tissues) resected by surgery. The correlation of DDX5 and E-cadherin expressions and the clinical pathological features of NSCLC were also analyzed. Results: The expression of DDX5 was higher in NSCLC compared with the adjacent normal tissues(63.5% vs 30.6%)(P<0.05). The expression of E-cadherin was lower in NSCLC compared with the adjacent normal tissues (60.8% vs 100%)(P<0.05). The expressions of DDX5 and E-cadherin in NSCLC were related to the lymph node metastasis and pathological stage of the tumor(P<0.05). But there was no significant correlation with the age, sex and pathological type of NSCLC(P>0.05). A negative corre- lation was found between the expression of DDX5 and E-cadherin(r=-0.327, P<0.05). Conclusion: Overexpression of DDX5 and down-regulation of E-cadherin might be involved in the development of NSCLC, DDX5 and E-cadherin might interact mutually.