在体单向肠灌流模型研究白芷中三种成分的吸收特性

目的:研究白芷中有效成分欧前胡素、异欧前胡素和花椒毒酚在大鼠小肠各段的吸收特征。方法:采用大鼠在体单向肠灌流模型结合HPLC法同时测定肠灌流液中欧前胡素、异欧前胡素和花椒毒酚含量的变化,考察高、中、低三组剂量下三种成分在十二指肠、空肠和回肠的吸收特性。结果:三种成分随着剂量的增大,吸收速率常数(Ka)和有效渗透系数(Peff)逐渐增大。三种成分相同剂量下各肠段间吸收均无显著性差异,其吸收大小顺序为:欧前胡素异欧前胡素花椒毒酚。结论:白芷三种主要成分在各肠段均吸收良好,吸收大小呈剂量相关性。

Intestinal Absorption of Three Components in Angelicae Dahuricae by in situ Single Pass Intestinal Perfusion Model in Rat

ABSTRACT Objective: To investigate absorption mechanism of imperatorin, isoimperatorin and methoxsalen in radix of Angelicae dahuricae. Methods: In situ single pass intestinal perfusion model was established, HPLC was used to determine the change of the con- centration of imperatorin, isoimperatorin and xanthotoxol in different segments. The absorption parameters of these three analytes in dif- ferent intestinal segments were investigated. Results: With the increasing of doses for these three analytes, the absorption rate constant (K _a ) and effective permeability coefficient (P _eff ) were increased. There were no significant differences in different segments of intestine in rat. The absorption order was imperatorin, isoimperatorin and xanthotoxol. Conclusion: Radix of Angelicae dahuricae was easily ab- sorbed in the intestine, the degree of absorption was dose-dependent.