良性前列腺增生患者外周血Th17和Treg细胞比率的变化

目的:探讨良性前列腺增生患者外周血Th17和Treg细胞比率的变化。方法:选择33例良性前列腺增生患者及19例正常对照者为研究对象,采用流式细胞术检测和比较其外周血中T淋巴细胞亚群及Th17和Treg细胞占CD4~+T细胞的比率。结果:良性前列腺增生患者外周血Th17和Treg细胞占CD4~+T细胞的比率分别为1.58±0.71和1.76±0.83,Th17/Treg的比率为0.89±0.42。正常健康对照者外周血Th17和Treg细胞占CD4~+T细胞的比率分别为0.75±0.46和1.83±0.75,Th17/Treg的比率为0.41±0.32。良性前列腺增生患者外周血Th17占CD4~+T细胞的比率和Th17/Treg的比率明显高于正常健康对照者(P0.05)。结论:良性前列腺增生患者体内Th17细胞比率升高,Th17/Treg比率失衡,可能与良性前列腺增生的发生、发展有关。

Changes of Th17 and Treg Cell Ratio in the Peripheral Blood of Patients with Benign Prostatic Hyperplasia

ABSTRACT Objective: To investigate the ratio of Th17 cells and CD4+CD25-Foxp3+ regulatory T (Treg) cells in the peripheral blood of patients with benign prostatic hyperplasia (BPH). Methods: The peripheral whole blood was obtained from patients with BPH(n=33) and healthy controls (n=19). The percentage of Th17 and Treg cells were analyzed by flow cytometry. Results: The percentage of Th17 and Treg cells to CD4⁺T lymphocyte in patient with BPH was 1.58±0.71 and 1.76±0.83, respectively. The ratio of Th17 to Treg cells in patients with BPH was 0.89±0.42. The percentage of Th17 and Treg cells to CD4⁺T lymphocyte in the healthy controls were 0.75±0.46 and 1.83±0.75, respectively. The ratio of Th17 to Treg cells in the healthy controls was 0.41±0.32. The percentage of Th17 cells and ratio of Th17 to Treg cells were significantly increased in patients with BPH compared with healthy controls. Conclusion: The increased percentage of Th17 cells made the imbalance of Th17 to Treg cells in patients with BPH. The imbalance might be involved in the pathogenesis of BPH.