神经生长导向因子SLIT2抑制结肠癌细胞迁移的作用研究

目的:研究SLIT2基因对结肠癌细胞迁移能力的影响。方法:通过western blot技术检测SLIT2在各种结肠癌细胞系中的表达,采用小干扰RNA转染技术,在低转移细胞系RKO中沉默SLIT2基因表达,并采用PCR和western blot技术验证其干扰效率。采用质粒转染技术,在高转移细胞系LOVO中上调SLIT2基因表达后,通过Transwell迁移实验和划痕愈合实验检测SLIT2表达变化对结肠癌细胞系迁移能力的影响。结果:SLIT2在高转移细胞系LOVO和HCT116中表达明显低于低转移细胞系RKO和HT-29。在低转移细胞系RKO中敲减SLIT2后,Transwell结果显示细胞的迁移能力得到了明显增强(P0.05)。在高转移细胞系LOVO中过表达SLIT2后,细胞的划痕愈合能力和迁移能力则均受到明显抑制(P0.05)。结论:神经生长导向因子SLIT2的表达与结肠癌细胞的迁移潜能相关,体外实验表明SLIT2可抑制结肠癌细胞系LOVO、RKO的迁移能力。

Axon Guidance Cues Slit2 Inhibited the Migration Ability of Colorectal Cancer Cell Lines

ABSTRACT Objective: To explore the role of SLIT2 on the migration ability of different colorectal cancer (CRC) cell lines. Methods: Western blot were performed to detect the expression level of SLIT2 in several CRC cell lines. Based on the expression level of SLIT2, RKO and LOVO were selected for further functional test. siRNA targeting SLIT2 was transfected into RKO, followed by Transwell assay to test the changed migration ability it may induce. LOVO cells transfected with the plasmid containing the coding sequence of SLIT2 were then detected the effect of ectopic SLIT2 on cell migration. Results: SLIT2 silencing facilitated the migration of the RKO. And ectopic expression of SLIT2 distinctly suppressed the wound closure and migration ability of LOVO with high metastasis potential. Conclusion: SLIT2 may exert an inhibitory effect on the migration ability of CRCs.