富氢盐水对高压电烧伤大鼠微循环内红细胞流速及组织病理损伤的影响

目的:探讨富氢盐水对高压电烧伤大鼠微循环内红细胞流速及组织病理损伤的影响。方法:80只大鼠随机分为四组,分别为烧伤组、治疗组、对照组和阳性对照组,治疗组烧伤后给予富氢盐水治疗,阳性对照组给予罂粟碱溶液治疗。每组分成三个亚组用于血气分析,肺组织形态学观察和红细胞流动速度检测。结果:治疗组和阳性对照组大鼠肺组织湿干重比均显著低于烧伤组(P0.05),治疗组和阳性对照组大鼠肺组织湿干重比差异不显著(t=0.585,P0.05)。治疗组和阳性对照组大鼠生存时间均显著高于烧伤组(P0.05),治疗组和阳性对照组大鼠生存时间差异不显著(t=0.448,P0.05)。治疗组和阳性对照组氧分压均显著高于烧伤组(P0.05);治疗组和阳性对照组氧分压差异不显著(t=0.424,P0.05)。治疗组和阳性对照组二氧化碳分压均显著低于烧伤组(P0.05);治疗组和阳性对照组二氧化碳分压差异不显著(t=1.285,P0.05)。治疗组和阳性对照组大鼠红细胞流动速度分压均显著高于烧伤组(P0.05);治疗组和阳性对照组之间大鼠红细胞流动速度差异不显著(P0.05)。结论:富氢盐水能够促进烧伤大鼠微循环内红细胞的流动,改善微循环,恢复受损肺组织,在高压电烧伤患者的治疗方面具有较好的应用前景。

Effect of Hydrogen Rich Saline on the Blood Flow Velocity and Tissue Pathological Damage of Rats with High Voltage Electric Burn

ABSTRACT Objective: To investigate the effect of hydrogen rich saline on blood flow velocity and tissue pathological damage of rats with high voltage electric burn. Methods: 80 Wistar rats were randomly divided into four groups, respectively for the burn group, treatment group, control group and positive control group. Treatment group was given hydrogen rich saline, the positive control group was given papaverine solution. Each group was divided into three subgroups for blood gas analysis, lung tissue observation and measure of red blood cell flow velocity detection. Results: The ratio of wet to dry of lung tissue in treatment group and positive control group was significantly lower than that of the burn group (P<0.05), and the difference between treatment group and positive control group was not significant (t=0.585, P>0.05). The survival time of the treatment group and the positive control group were significantly higher than that of the burn group (P<0.05), and the difference of survival time between the two groups of treatment group and the positive control group was not significant (t=0.448, P>0.05). The oxygen partial pressure of the treatment group and the positive control group were significantly higher than that of the burn group (P<0.05); the difference of the oxygen partial pressure between the treatment group and the positive control group was not significant (P>0.05, t=0.424). The carbon dioxide partial pressure in the treatment group and the positive control group was significantly lower than that of the burn group (P<0.05); the difference of the carbon dioxide partial pressure between the treat- ment group and the positive control group was not significant (t=1.285, P>0.05). The flow velocity of red blood cells in the treatment group and the positive control group were significantly higher than that of the burn group (P<0.05); the difference of the red blood cell flow velocity between the treatment group and the positive control group was not significantly different (P>0.05). Conclusion: Hydrogen rich saline can promote the flow of red blood cells in the microcirculation, improve microcirculation, restore damaged lung tissue, and have a good prospect in the treatment of patients with high voltage electric burn.