益坤精胶囊对骨质疏松模型小鼠骨形态和血清中IL-6表达的影响

目的:观察益坤精胶囊对骨质疏松模型小鼠骨形态计量学指标及血清白介素-6(IL-6)的影响。方法:取60只C57雌性小鼠随机分为:模型组(等体积生理盐水)、雌激素组(尼尔雌醇,0.25 mg/kg)、益坤精胶囊高剂量组(1.44 g/kg)、中剂量组(0.72 g/kg)、低剂量组(0.36 g/kg)及假手术组(等体积生理盐水),各组灌胃均70 d。采用酶联免疫吸附测定(ELISA)法检测血清中IL-6浓度,BI-2000医学图像分析系统进行骨形态计量学指标检测,使用CT测量分析各组小鼠股骨远侧干骺端血管数量。结果:与假手术组比较,模型组骨小梁平均宽度、骨皮质平均厚度、骨小梁面积、成骨细胞数以及骨密度、骨血管数量均明显减小,而破骨细胞数、血清中IL-6的浓度明显上升(P0.05)。与模型组比较,雌激素组、益坤精胶囊高、中、低剂量组上述指标均明显改善(P0.05),且益坤精胶囊高剂量组与雌激素组疗效相当(P0.05)。结论:益坤精胶囊可明显改善骨质疏松模型小鼠的骨形态计量学指标,降低血清中IL-6水平,增加骨血管数量,且以1.44 g/kg益坤精胶囊灌胃效果最佳。

Effects of Yikunjing Capsules on the Bone Histomorphometry Indexes and Serum IL-6 in Mice with Osteoporosis

ABSTRACT Objective: To observe the effects of Yikunjing capsules on the bone histomorphometry indexes and the serum Interleukin-6(IL-6) level in mice with osteoporosis. Methods: 60 cases of C57 female mice were randomly divided into 5 groups, such as model group (equal volume of normal saline), estrogen group (nilestriol, 0.25 mg/kg), Yikunjing capsules high dose group (1.44 g/kg),Yikunjing capsules medium dose group (0.72 g/kg) and Yikunjing capsules low dose group (0.36 g/kg), sham group (equal volume of normal saline) were treated with sham operation. The mice in each group was intragastrically administrated for 70 days. Enzyme linked immunosorbent assay (ELISA) was used to detect the serum IL-6 level. The bone histomorphometry index were detected with BI-2000 Medical Image Analysis System. And the Number of blood vessels in distal femoral metaphysis of each group was measured by CT. Results: Compared with the sham group, the width and area of trabecular bone,the thickness of cortical bone, area of trabecular bone, the number of osteoblasts, the bone mineral density and the number of blood vessels in model group were significantly reduced(P<0.05),while the number of osteoclasts and serum IL-6 level were significantly increased(P<0.05). Compared with the model group, above indexes in estrogen group, Yikunjing capsules high, medium, low dose group were improved(P<0.05), and the effect of Yikunjing capsules high dose group and estrogen group was almost the same(P>0.05). Conclusion: Yikunjing capsules can rectify the bone histomorphometry indexes reduce the serum IL-6 level and increase the number of blood vessels in mice with osteoporosis, and intragastrically administrating 1.44 g/kg Yikunjing capsules could get the better effect.