转录因子二聚化配体3（TFDP3）在乳腺癌组织中的表达及其与乳腺癌分子分型的相关性研究

目的:本研究旨在分析总结癌-睾丸抗原TFDP3在乳腺癌中的表达规律,探究TFDP3表达与乳腺癌分型及发病进程关系。方法:通过对不同分型及分期的乳腺癌组织切片进行免疫组化检测,分析TFDP3在其中的表达情况,并结合样本来源患者的临床信息,对TFDP3的表达与乳腺癌分子分型、分期及预后的相关性进行统计分析。同时,通过Western Blot检测了5种乳腺癌细胞系(MDA-MB-231、MCF-7、SK-BR-3、T47D及MCF-10A)中TFDP3的表达情况,并通过细胞免疫荧光实验,检测TFDP3在细胞中的定位。结果:TFDP3在已检测的乳腺癌样本中的表达率为49%。乳腺癌临床分子分型标记物HER2的表达与TFDP3的表达呈正相关,但乳腺癌的分期、临床病理分型以及临床分子分型标记物ER、PR的表达均与肿瘤组织中TFDP3的表达无相关性。结论:TFDP3在各在乳腺癌组织中高表达,其表达量与HER2的表达量呈正性相关。这为研究已TFDP3为靶点的乳腺癌免疫治疗,提供了新的依据。

The Expression of TFDP3 in Breast Cancer and Its Role of Breast Cancer Molecular Classification

ABSTRACT Objective: The object of this study is to analysis the expression of cancer testis antigen TFDP3 in breast cancer, to ex- plore the correlation between TFDP3 and development of breast cancer. Methods: The expression of TFDP3 was detected in pathological section of tumor tissues from patients with breast cancer using immunohistochemical method. And we analyzed the data with clinical in- formation of patients to discover the correlation of TFDP3 and the molecular classification of breast cancer. We also detected the expres- sion of TFDP3 protein in five breast cancer cell lines, including MDA-MB-231, MCF-7, SK-BR-3, T47D and MCF-10A, using Western Blot. Then we verified the location of TFDP3 protein in cells through immunofluorescence method. Results: TFDP3 is greatly and broadly expressed in many cell lines and breast cancer tissues, such as breast, lymph node, hypothyroid, appendix and so on, while TFDP3 is 49% percent positive in breast cancer. The expression of TFDP3 is unrelated with clinical stage, pathology type and molecular classification of breast cancer, but related with special tumor marker HER2. Conclusion: TFDP3 highly expresses in breast cancer is related with the ex- pression of HER2. It suggests that TFDP3 be a new potential target molecule of immunotherapy for breast cancer.