莫沙必利对糖尿病胃轻瘫大鼠胃窦Cajal间质细胞的影响

目的:研究莫沙必利对糖尿病胃轻瘫大鼠胃窦Cajal间质细胞(ICC)的影响。方法:将48只SD雄性大鼠随机分为正常组、模型组、莫沙必利组,各组分别16只。模型组、莫沙必利组大鼠给予一次性腹腔注射链脲佐菌素后建立糖尿病胃轻瘫模型,建模第10天起,莫沙必利组大鼠给予莫沙必利注灌胃治疗。建模30周后,检测各组大鼠的血糖水平、胃残留率,通过采用电生理学方法检测各组大鼠胃动力,并采用免疫组化染色方法检测各组胃窦ICC的相对数量。结果:模型组大鼠胃残留率明显高于正常组大鼠(P0.05),而给予莫沙必利后胃残留率显著降低(P0.05);模型组大鼠胃电图波幅和频率均明显低于正常组大鼠(P0.05),而给予莫沙必利后胃电图波幅和频率明显增加(P0.05);模型组大鼠胃窦ICC明显低于正常组大鼠(P0.05),而给予莫沙必利后胃窦ICC有所增加(P0.05)。结论:莫沙必利可显著改善糖尿病胃轻瘫大鼠的胃动力,可能与其增加胃窦Cajal间质细胞有关。

Effect of Mosapride on the Interstitial Cells of Cajal in Gastric Antrumof Diabetic Gastroparesis Rats

Objective:To study the effects of mosapride on the gastroparalysis interstitial cells of Cajal in the gastric antrum of diabetic rats.Methods:48 male SD rats, were randomly divided into normal group, model group, mosapride group, 16 rats each group. Model group, mosapride rats given intraperitoneal injection of streptozotocin (STZ) after the establishment of diabetic gastroparesis model, Modeling the 10th day onwards, mosapride rats administered orally mosapride. Modeling after 30 weeks, blood glucose levels of the rats, gastric residual rate of detection of gastric motility of rats by using electrophysiological methods, and by immunohistochemical staining method to detect the relative amount of each group relative number of ICC.Results:Gastric residual rate of rats was significantly higher than normal group (P <0.05), but after giving mosapride gastric residual rate decreased significantly (P <0.05); EGG amplitude and frequency of model group were significantly lower than normal group (P <0.05), but after giving mosapride EGG amplitude and frequency was significantly increased (P <0.05); Antral ICC model group was significantly lower than normal group (P <0.05), but after giving mosapride antral ICC increased (P <0.05).Conclusion:Mosapride significantly improved diabetic gastroparesis gastric motility in rats may be related to increased inter-related antral cells of Cajal.