人巨细胞病毒IE1-72蛋白在胶质瘤中的表达及意义

目的:探讨人巨细胞病毒(HCMV)立刻早期基因1-72(IE1-72)蛋白在胶质瘤中的表达水平以及HCMV感染与胶质瘤发生的病因学关系。方法:采用免疫组化方法检测HCMV IE1-72蛋白在125例人脑胶质瘤组织及10例正常人脑组织中的表达,分析其表达水平与胶质瘤临床病理学特征的关系。结果:IE1-72蛋白在胶质瘤组织中的表达水平明显高于正常人脑组织(P=0.000);IE1-72蛋白免疫染色强度随胶质瘤病理级别的升高而明显增强(r=0.310,P=0.000),其在高恶性度胶质瘤中的染色强度明显强于低恶性度胶质瘤(P=0.004);IE1-72蛋白染色强度与胶质瘤患者的年龄存在正相关(r=0.234,P=0.009),而与胶质瘤患者的性别(r=0.038,P=0.675)以及肿瘤部位(r=0.086,P=0.341)无明显相关性。结论:HCMV感染及其蛋白IE1-72表达可能与人脑胶质瘤的发生和发展密切相关,但其确切的致瘤机制尚需进一步研究。

Expression and Significance of IE1-72 Protein of Human Cytomegalovirus in Gliomas

ABSTRACT Objective: To investigate the expression of immediate early gene 1-72 (IE1-72) protein of human cytomegalovirus (HCMV) in gliomas and the etiological relationship between HCMV infection and glioma tumorigenesis. Methods: The expression of IE1-72 protein of HCMV was detected by immunohistochemistry method in 125 cases of human brain glioma and 10 cases of normal human brain tissue, as well as the correlation between its expression level and clinicopathological features of gliomas was further analyzed. Results: The expression level of IE1-72 protein in gliomas was significantly higher than that in normal human brain tissues (P=0.000). IE1-72 immunoreactivity intensity was enhanced significantly with gliomas pathological grade ascending (r=0.310, P=0.000), and which in malignant glioma tissues was significantly higher than that in benign glioma tissues (P=0.004). IE1-72 immunoreactivity intensity showed a significantly positive correlation with age of glioma patients (r=0.234, P=0.009), however no obvious correlation with gender (r=0.038, P=0.675) and tumor location (r=0.086, P=0.341) of glioma patients. Conclusion: HCMV infection and its protein IE1-72 expression may be closely associated with the initiation and development of human brain gliomas, however the exact oncogenous mechanism need to be researched further.