MMP-9基因多态性与缺血性脑卒中发病及预后的相关性研究

目的:研究基质金属蛋白酶9(MMP-9)基因多态性与缺血性脑卒中(IS)发病及预后的相关性,为IS的防治提供新的理论依据。方法:选取治疗的IS患者100例,根据TOAST分型标准分为大动脉粥样硬化型(LAA)组41例,小动脉阻塞型(SAO)组59例,并选取健康体检者40例作为对照组,采用PCR-RFLP法检测各组MMP-9基因C1562T、R279Q多态性,并对IS患者进行3个月的随访,采用Logistic回归分析C1562T、R279Q多态性与IS患者预后的相关性。结果:LAA组、SAO组MMP-9基因C1562T位点T等位基因、C/T+T/T基因型频数均高于对照组,差异有统计学意义(P0.05),LAA组、SAO组C1562T位点C等位基因、C/C基因型频数及R279Q位点等位基因和基因型频数与对照组比较差异无统计学意义(P0.05);Logistic回归分析显示,MMP-9各型别基因与预后无明显相关性(P0.05)。结论:MMP-9基因C1562T的T等位基因是IS发病的穿易感基因之一,但MMP-9基因多态性与IS患者的预后并无明显相关性。

Correlation of MMP - 9 Gene Polymorphism with the Pathogenesis and Prognosis of Patients with Ischemic Stroke

ABSTRACT Objective: To study the relation of MMP-9 gene polymorphism with the pathogenesis and prognosis of patients with IS, so as to provide a new theoretical basis for IS prevention. Methods: The selected 100 patients were divided into the LAA group (41 cases), and the SAO group (59 cases ) according to TOAST classification standard. Another 40 healthy people was selected as the control group. PCR-RFLP method was applied to detect their MMP-9 gene C1562T and R279Q polymorphism. All patients were followed up for 3 months. Logistic regression model was applied to analyze the relationship betwen C1562T and R279Q polymorphism and prognosis of IS patients. Results: MMP-9 C1562T gene loci alleles T, C/T + T/T genotype frequency in the LAA group and the SAO group were higher than in the control group, and the difference was statistically significant (P < 0.05). C1562T loci alleles C, C/C genotype frequency and R279Q loci alleles and genotypes frequency of LAA group and SAO group had no statistical difference when compared with that of the control group (P>0.05). Multi-factor unconditioned Logistic regression analysis showed that all genetypes of MMP-9 had no obvious correlation with prognosis (P>0.05). Conclusion: MMP-9 C1562T T allele was one of the susceptibility genes to IS, but MMP-9 gene polymorphism had no significant correlation with the prognosis.