三棱内酯B调控人冠状动脉内皮细胞基因表达谱的生物信息学分析

目的:分析三棱内酯B在人冠状动脉内皮细胞中的表达谱数据集,寻找三棱内酯B调控血管内皮功能的关键作用靶点。方法:基于GEO公共数据库,下载原始表达谱数据集(GSE44598),经过差异基因筛选,功能注释,通路富集,信号通路网络以及基因互作网络分析,找出三棱内酯B对人冠状动脉内皮细胞基因表达谱产生影响的关键基因和信号通路。结果:同对照组相比,三棱内酯B给药组共有5224个基因有显著性差异,包括2628个上调基因和2596个下调基因。基因功能注释和信号通路富集分析表明,差异基因主要参与了细胞周期过程。网络分析显示,MAPK信号通路、细胞周期通路以及PLCG2,PRKACA和ADCY4等为关键信号通路和基因。结论:三棱内酯B通过影响PLCG2,PRKACA,ADCY4等基因的表达,参与MAPK和细胞周期等信号通路,从而调节人冠状内皮细胞的功能。这些关键基因和信号通路是三棱内酯B在心血管疾病治疗应用中潜在的作用靶点。

Bioinformatics Analysis on Gene Expression Profile Alterations of HCAEC Regulated by Sparstolonin B

ABSTRACT Objective: To find the key therapeutic targets involved in Sparstolonin B-regulated human coronary arterial endothelial cells (HCAECs) by using a bioinformatics analysis. Methods: Based on the GEO public database, the original expression profile data (GSE44598) were obtained, and a series of analysis, including differential expression gene screening, function annotation, pathway cluster- ing, pathway-net and signal-net analysis were conducted to find the key genes and signaling pathways related to Sparstolonin B. Results: Compared with control group, 5224 genes were found in Sparstolonin B group, including 2628 up-regulated genes and 2596 down-regu- lated genes, respectively. Gene function annotation and signaling pathway enrichment analysis manifested that the differently expression genes were primarily involved in cell cycle regulation process. Network analysis indicated that the MAPK signal pathway, cell cycle sig- nal pathway and PLCG2, PRKACA and ADCY4 were the key pathways and genes. Conclusion: Our findings revealed that Sparstolonin B played a key role in regulating the function of HCAEC by affecting the expression of many genes, like PLCG2, PRKACA and ADCY4, which were involved in MAPK and cell cycle signaling pathways. These key genes and pathways were identified as potential therapeutic targets of Sparstolonin B on cardiovascular disease.