小口径血液导流管的实验研究

目的:探讨小口径血液导流管在动物离断肢体模型中快速恢复通血的实验基础应用,研究小口径血液导流管实验幼猪离断肢体维持通血效果的评价。方法:20只实验幼猪随机分为A、B两组,制成后肢完全离断模型模型,采用内径为2.0 mm、外径2.5mm的血液导流管,A组长度10 cm;B组长度20 cm,进行血管桥接后定期观察血液导流管通畅性,观察终点为血液导流管完全堵塞,血管超声探测仪无血流信号,远端血管搏动消失,离断肢体以远皮下毛细血管网无渗血。比较两组到达观察终点的时间有无差异。结果:建立临时血管通路后,离断肢体远端股动脉的远端有搏动,血管超声探测仪可检测到血液导流管内有血流信号,随着时间的延长,血液导流管动脉段逐渐由鲜红色变为暗红色,导流管段逐渐形成附壁血栓,远端血管搏动及皮下毛细管网渗血逐渐减弱直至消失,血流信号消失,两组到达观测终点的时间分别为A组365±47.4 min;B组359±31.5 min,两者比较其差异没有统计学意义(P0.05)。说明长度在10 cm-20 cm的小口径血液导流管在实验动物离断肢体血管通血方面无明显差异。结论:小口径血液导流管能够用于动物离断肢体的血管临时桥接,维持通血时间可达6-8小时,有效通血时间长。实验数据说明小口径血液导流管适合于动物离断肢体模型中的血管桥接,在下一步临床应用中在四肢复杂血管损伤中有着较为广阔的临床应用前景。

The Experiment Study of Small Diameter Intravascular Catheter

ABSTRACT Objective: To explore the the experimental application of small diameter intravascular catheter using on animal amputated limb model in order to quickly recover blood supply and to discuss the evaluation of small diameter intravascular catheter using in experimental piglets. Methods: There are 20 experimental piglets which are divided into groups A and B randomly. They are established to amputated limb model using the small diameter intravascular catheter of inner diameter of 2.0 mm and outside diameter 2.5 mm. Length in group A is 10 cm and group B 20 cm. To observe the patency after Vascular bridge connection. The observation endpoint with the intravascular catheter completely blocked were as follow: no blood flow signal on Doppler ultrasound and no pulse in distal blood vessel and distal amputated limb without bleeding. Comparing the differences of time endpoint of two groups differences. Results: After established temporary vascular access, the distal femoral artery of amputated limb start to pulse and vascular Doppler ultrasound can detect the signal of blood flow. With the extension of time, the color of diameter intravascular catheter changed from red to dark red with the thrombus formation in inner catheter. The pulse of distal vascular are gradually weakened. The exudation of subcutaneous blood capillary net are also disappeared. The time endpoint of two groups are respectively group A (365±47.4 min) and group B (359± 31.5 min). Compared with the differences between two group, it has no statistical significance (P > 0.05). To show that small diameter intravascular catheter (length from 10 cm to 20 cm) in experimental animals have not obvious differences with Vascular bridge connection. Conclusion: Small diameter intravascular catheter can be used in animal amputated limb model in order to bridge vascular temporarily. It can maintain blood shunt from six to eight hours. And effective time is longer than others. Experimental data shows that small diameter intravascular catheter is suitable for animal amputated limb model in vascular bridge connection. In the next step, it can be widely used into the complicated injury of amputated limb in clinical application.