| 云芝糖肽在ROS、p3 8信号通路中对巨噬细胞Raw264.7的免疫调节作用 |
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| 引用本文： | 董莎莎,杨晓彤,冯慧琴,杨明俊,杨庆尧.云芝糖肽在ROS、p3 8信号通路中对巨噬细胞Raw264.7的免疫调节作用[J].现代生物医学进展,2011,11(15):2848-2850. |
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| 作者姓名： | 董莎莎 杨晓彤 冯慧琴 杨明俊 杨庆尧 |
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| 作者单位： | 1. 上海师范大学微生物与免疫学研究所,上海,200234
2. 上海芝草生物技术有限公司,上海,200234 |
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| 摘 要： | 目的:探究云芝糖肽(PSP)对静息和脂多糖(LPS)过度刺激两种不同状态的巨噬细胞Raw264.7产生活性氧簇(ROS)及p38、p-p38的影响及其相关机制。方法:采用流式细胞术方法检测ROS的表达;Western blot方法检测MAPK信号通路中p38磷酸化及非磷酸化的相对表达量。结果:100g/ml PSP下调1g/ml LPS单独作用于巨噬细胞时ROS及p-p38的表达量;100g/mlPSP上调正常巨噬细胞p-p38的表达量;p38表达量基本不变。结论:PSP通过ROS,p38两条通路参与巨噬细胞的免疫调节作用。
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| 关 键 词： | 云芝糖肽(PSP) 脂多糖(LPS) 巨噬细胞 炎症 ROS p38 |
Immunomodulatory Effects of Coriolus Versicolor Polysaccharopeptide on Macrophages Raw264.7 in ROS and p38 Signaling Pathways |
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| DONG Sha-sha,YANG Xiao-tong,FENG Hui-qin,YANG Ming-jun,YANG Qing-yao.Immunomodulatory Effects of Coriolus Versicolor Polysaccharopeptide on Macrophages Raw264.7 in ROS and p38 Signaling Pathways[J].Progress in Modern Biomedicine,2011,11(15):2848-2850. |
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| Authors: | DONG Sha-sha YANG Xiao-tong FENG Hui-qin YANG Ming-jun YANG Qing-yao |
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| Institution: | DONG Sha-sha~1,YANG Xiao-tong~1,FENG Hui-qin~1,YANG Ming-jun~1,YANG Qing-yao~2 (1 Institutes of Microbiology and Immunology,Shanghai Normal University,SHANGHAI 200234,China,2 Shanghai Zhi-Herb Biotechnology Co.,Ltd.,China) |
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| Abstract: | Objective:To investigate the effects and mechanisms of Coriolus versicolor polysaccharopepide(PSP) on the production of Reactive oxygen species(ROS),p38 and phosphor-p38 on normal and lipopolysaccharides(LPS) stimulated murine macrophage.Methods:ROS was measured by Flow cytometry;p38and phosphor-p38 were detected by Western blot.Results:While stimulated by LPS(1g/ml),there was less ROS and phosphor-p38 in cells pretreated with PSP(100g/ml);100g/ml PSP along could increase the expression of phosphor-p38;The ... |
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| Keywords: | PSP LPS Macrophages Inflammation ROS p38 |
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