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| Fra-1对大鼠C6胶质瘤细胞侵袭转移能力的影响 | |
| 引用本文： | 田刚,王晓娟,雷宁,曾庆乐,樊明新,徐英,杨江河,卢宁,李华.Fra-1对大鼠C6胶质瘤细胞侵袭转移能力的影响[J].现代生物医学进展,2011,11(16):3038-3040. |
| 作者姓名： | 田刚 王晓娟 雷宁 曾庆乐 樊明新 徐英 杨江河 卢宁 李华 |
| 作者单位： | 1. 兰州军区乌鲁木齐总医院神经外科 新疆乌鲁木齐 830000 2. 解放军第23医院临床部药械科 新疆乌鲁木齐 830000 3. 成都理工大学 材料与化学化工学院 化工与制药系 四川成都610059 4. 兰州军区乌鲁木齐总医院肿瘤科 新疆乌鲁木齐 830000 |
| 摘 要： | 目的:观察Fra-1对C6胶质瘤侵袭转移能力的影响。方法:以大鼠C6胶质瘤细胞为研究对象,Fra-1 siRNA通过脂质体转染C6,realtime RT-PCR和western blot法检测C6细胞中Fra-1的表达、Elisa法检测细胞培养上清中MMP-9的含量,Transwell小室观察C6细胞的转移侵袭能力。结果:干扰Fra-l后能降低C6细胞中Fra-1的表达,显著降低C6细胞中MMP-9的含量,降低C6细胞的转移侵袭能力。结论:干扰Fra-1能抑制C6细胞的转移侵袭。 |
| 关 键 词： | Fra-1 基质金属蛋白酶 C6 |
Effects of Fra-1 on Invasive Ability of C6 Glioma Cells |
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| TIAN Gang,WANG Xiao-juan,LEI Ning,ZENG Qing-le,FAN Ming-xin,XU Ying,YANG Jiang-he,LU Ning,LI Hua.Effects of Fra-1 on Invasive Ability of C6 Glioma Cells[J].Progress in Modern Biomedicine,2011,11(16):3038-3040. | |
| Authors: | TIAN Gang WANG Xiao-juan LEI Ning ZENG Qing-le FAN Ming-xin XU Ying YANG Jiang-he LU Ning LI Hua |
| Institution: | TIAN Gang~1,WANG Xiao-juan~2,LEI Ning~3,ZENG Qing-le~3,FAN Ming-xin~1,XU Ying~1,YANG Jiang-he~1,LU Ning~4,LI Hua~1 (1 Department of neurosurgery of Urumchi General Hospital in Lanzhou Military Area Command,Urumchi 830000,2 Department of Plant Protection Machine,The 23th hospital of Chinese People's Liberation Army,3 Department of Chemical & Pharmaceutical Engineering,College of Material and Chemistry & Chemical Engineering,Chengdu University of Technology.Chengdu 610059,4 Department of medi... |
| Abstract: | Objective:To study the effect of Fra-1 on invasive ability of C6 cells.Methods:C6 cells were divide into blank group, control group,Fra-1 siRNA groups.The effects of Fra-1 siRNA on C6 cells invasive ability in vitro was measured by Boyden chamber invasive models.Contents of Fra-1 protein and mRNA were detected by western blot and realtime RT-PCR,respectively.Contents of MMP-9 were measured by ELISA methods.Results:In siRNA group,contents of Fra-1 and MMP-9 were decreased markedly,the invasive ability was de... |
| Keywords: | Fra-1 Matrix metalloproteinase C6 |
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