兔颈动、静脉移植桥血管狭窄模型的建立及其电镜观察

目的:建立兔颈动、静脉移植血管桥动物模型,观察移植桥血管内膜增生和狭窄的电镜下表现。方法:通过兔双侧颈动脉进行动脉桥和静脉桥的移植,形成双侧移植血管桥再狭窄动物模型。在第8周施行血管桥移植手术的同时留取右侧颈动静脉标本作为对照血管,再分别于第12周、16周和第20周分别处死模型兔,采集移植桥血管标本,在光镜下测量其内膜厚度、面积、狭窄度,并进行电镜观察。结果:颈动脉和颈静脉桥移植后,随着时间的延长,桥血管的出现平滑肌迁移,脂质沉积,内膜增生,血管狭窄等改变,且以静脉桥血管的病理改变更为明显。结论:在兔形成动脉粥样硬化病变基础上,进行双侧颈动脉血管桥的移植,建立兔双侧颈动脉移植血管桥再狭窄动物模型,有利于设立自身对照,研究术后动静脉桥再狭窄差异机制;建立动、静脉桥后,位于血管中膜的平滑肌细胞出现向血管内膜迁移现象,说明中膜平滑肌细胞迁移进入内膜导致新内膜形成是血管再狭窄的重要环节。

Construction of a Vascular Graft Atherosclerotic and Stenotic Rabbit Model and Its Observation Under Scanning Electron Microscope

Objective:To construct a vascular graft atherosclerotic and stenotic rabbit model and to observe the intimal hyperplasia and stenosis of the transplant graft under scanning electron microscope.Methods:Studies were performed by using New England rabbits,weight 2-2.5kg,animals were fed with high fat diet to mimic the pathology of coronary atherosclerosis in human for 8 weeks,and to subjected to premedication,anesthesia.A longitudinal incision was made on the median of neck to expose bilateral carotid arteries...