| 过表达ER恢复雌激素对子宫内膜癌KLE细胞中Notch信号通路的调控 |
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| 引用本文： | 魏桠楠,周冬梅,吴凌,杨婷,祝颖,冯怡雯,张箴波,祝亚平,丰有吉.过表达ER恢复雌激素对子宫内膜癌KLE细胞中Notch信号通路的调控[J].现代生物医学进展,2011,11(16):3001-3004. |
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| 作者姓名： | 魏桠楠 周冬梅 吴凌 杨婷 祝颖 冯怡雯 张箴波 祝亚平 丰有吉 |
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| 作者单位： | 上海交通大学附属上海市第一人民医院 上海 200080 |
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| 基金项目： | 国家自然科学基金，上海市卫生局面上项目 |
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| 摘 要： | 目的:通过观察雌激素对子宫内膜癌KLE细胞中Notch信号通路的影响,探讨过表达雌激素核受体（estrogen receptor,ER）是否可以恢复雌激素对Notch信号通路的调控作用,继而调节细胞增殖活性。方法:MTT检测雌激素及Notch信号通路对细胞增殖活性的影响;RT-PCR及Western-blotting检测雌激素及Notch通路抑制剂DAPT对Notch表达的影响;质粒的抽提及转染使KLE细胞中的雌激素核受体ER过表达。结果:雌激素呈剂量依赖效应促进KLE细胞的增殖活性,其中以雌激素浓度为1.0×10-9M时最明显（相对于对照组为1.25±0.026,P<0.05）;抑制Notch信号通路的表达可以明显下调KLE细胞的增殖活性（0.76±0.02,P<0.05）;在KLE细胞中,雌激素对Notch的表达没有明显的调控作用,但是将其雌激素核受体过表达后,雌激素可明显上调Notch的表达,并显著促进细胞的增殖活性（1.24±0.02,P<0.05）。结论:在ER阴性的子宫内膜癌细胞中过表达ER,可以恢复雌激素对Notch信号通路的调控,从而进一步的调控细胞增殖活性。
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| 关 键 词： | KLE 雌激素 Notch信号通路 增殖活性 核受体 |
Effect of Extra-expression of Estrogen Receptor in Restoration of Regulation of Estrogen on Notch Signaling Pathway in Endometrial Cancer Cells |
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| WEI Ya-nan,ZHOU Dong-mei,YANG Ting,WU Ling,ZHU Ying,FENG Yi-wen,ZHANG Zhen-bo,ZHU Ya-ping,FENG You-ji.Effect of Extra-expression of Estrogen Receptor in Restoration of Regulation of Estrogen on Notch Signaling Pathway in Endometrial Cancer Cells[J].Progress in Modern Biomedicine,2011,11(16):3001-3004. |
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| Authors: | WEI Ya-nan ZHOU Dong-mei YANG Ting WU Ling ZHU Ying FENG Yi-wen ZHANG Zhen-bo ZHU Ya-ping FENG You-ji |
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| Institution: | (Department of Obstetrics and Gynecology,First Hospital,Shanghai jiaotong University,Shanghai 200080,China) |
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| Abstract: | Objective:To observe the effect of estrogen on Notch signaling pathway in endometrial cancer cells,and to explore whether estrogen can induce the expression of Notch when estrogen receptor(ER) is up-regulated,further affecting the cell proliferation. Methods:MTT was used to measure the proliferative activity after various treatments.RT-PCR and Western-blotting were used to determine the effect of estrogen and DAPT on Notch expression in mRNA and protein levels.Plasmid was transfected into KLE to induce the ... |
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| Keywords: | KLE Estrogen Notch Proliferative activity Estrogen receptor |
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