| Neurospora crassa phytase功能区基因phy A'的克隆表达及活性变化 |
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| 引用本文： | 周小玲,黄天华,诸葛健.Neurospora crassa phytase功能区基因phy A''的克隆表达及活性变化[J].现代生物医学进展,2008,8(11). |
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| 作者姓名： | 周小玲 黄天华 诸葛健 |
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| 作者单位： | 1. 汕头大学医学院,广东,汕头,515041
2. 江南大学生物工程学院,江苏,无锡,214036 |
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| 基金项目： | 汕头大学博士后科研启动基金 |
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| 摘 要： | 目的:检测N.crassa phtase功能区基因phy A′表达的Phy A′活性变化。方法:先将N.crassa phy A′克隆到表达载体pPIC9K中,然后将获得的重组质粒pPIC9K-phy′转化到毕赤氏酵母中分泌表达,分离纯化重组酶Phy A′,并测定其酶学性质。结果:与原酶相比,N.crassa Phy A′的最适温度降低、热稳定性降低以及酶活力的降低。结论:N.crassa phtase N端的前12个氨基酸对维持该酶的功能有比较重要的作用。
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| 关 键 词： | 植酸酶 功能区基因 活性 |
Clone, Expression and Activity Change of Neurospora crassa Phytase phy A' Motif |
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| ZHOU Xiao-ling,Huang Tian-hua,Zhuge Jian.Clone, Expression and Activity Change of Neurospora crassa Phytase phy A'' Motif[J].Progress in Modern Biomedicine,2008,8(11). |
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| Authors: | ZHOU Xiao-ling Huang Tian-hua Zhuge Jian |
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| Institution: | ZHOU Xiao-ling~1 Huang Tian-hua~1 Zhuge Jian~2 |
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| Abstract: | Objective:To investigate the enzyme activity of N.crassa phytase phy A'motif.Methods:N.crassa phtase phy A' was cloned into the expression vector pPIC9K.Then,the recombinant plasmid pPIC9K- phy' was transformed into Pichia pastoris KM71 to express and purify the recombinant enzyme Phy A'.And the activity of Phy A' was determined.Results:The temperature optimum,ther- mostability and activity of Phy A' decreased compared with N.crassa phrase.Conlusions:The 12 amino acids of N-terminal play an im- portant role in maintaining the activity of N.crassa phytase. |
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| Keywords: | Phytase Motif Activity |
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